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Humanized glioma PDX mouse model as well as preparation method and application thereof

A mouse model, glioma technology, applied in the field of tumor, can solve the problems of inability to fully evaluate anti-tumor efficacy, lack of immune system, etc.

Active Publication Date: 2021-06-11
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention provides a humanized glioma PDX mouse model to solve the problem that the usual tumor model uses immunodeficiency animals to transplant human tumor cells or tissues. Due to the lack of a complete immune system, the anti-tumor efficacy cannot be completely evaluated. technical problem

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  • Humanized glioma PDX mouse model as well as preparation method and application thereof
  • Humanized glioma PDX mouse model as well as preparation method and application thereof
  • Humanized glioma PDX mouse model as well as preparation method and application thereof

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preparation example Construction

[0030] The preparation method of the humanized glioma PDX mouse model of the present embodiment comprises the following steps:

[0031] S1. Construction of glioma model in nude mice: Implantation of patient-derived glioma cells into mice;

[0032] S2. Culture: Raise mice until the volume of glioma cells grows to 62mm 3 ~100mm 3 ;

[0033] S3. Construction of humanized glioma PDX mouse model: irradiate mice with sublethal dose of X-rays, transplant homologous functional lymphocytes, and detect the growth of glioma cells and lymphocytes in mice The proliferation and differentiation of humanized glioma PDX mice were obtained.

[0034] The preparation method of humanized glioma PDX mouse model, expressing the patient's immune system while loading human glioma, this model provides tumor cells with a growth microenvironment more similar to that of the human body, and can be studied from histopathology, gene The expression, gene mutation, inflammation and treatment response truly...

Embodiment 1

[0046] The preparation method of humanized glioma PDX mouse model comprises the following steps:

[0047] S1. Construction of glioma model in nude mice: preparation of NRG (NOD.CgRag1 tmiMom IL2rg tm1wjl / Sz) mice, centrifuge the tumor sphere culture of fresh surgical specimens from the patient at a centrifugal force of 200×g for 5 minutes, digest the precipitate into single cells with Accutase cell digestion solution, and prepare again in ice-cold PBS The cell suspension is ready for use, and the concentration of the cell suspension is 5×10 7 / ml, drill the skull at 1 mm to 2 mm lateral to the intersection of the midline and the coronal suture and 1 mm in front of the coronal suture, inhale the cell suspension into the Hamilton syringe and needle on the stereotaxic instrument, and aim the needle at the center of the skull hole before starting the injection Wait for 1 min, inject the cell suspension into the mouse brain at a speed of 1 μL / min, the injection volume of the ce...

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Abstract

The invention discloses a humanized glioma PDX mouse model. The humanized glioma PDX mouse model is obtained by transplanting human glioma cells and functional lymphocytes homologous to the glioma cells. The humanized glioma PDX mouse model disclosed by the invention is obtained by transplanting the human glioma cells and the functional lymphocytes homologous to the glioma cells. The lymphocytes are transplanted into the body of an immunodeficient mouse, so that the mouse has a human immune function, the mouse can express the immune system of a patient while loading human glioma, and the interaction between the human tumor cells and the immune system can be simulated; and the mouse model has an important application prospect in the aspects of research and development and preclinical evaluation of tumor immunotherapy drugs. Moreover, the humanized glioma PDX mouse model has relatively high T cell induced differentiation capability, and an important research tool is provided for immunotherapy of glioma and research and development of new drugs and new equipment.

Description

technical field [0001] The present invention relates to the field of tumors, in particular to a humanized glioma PDX mouse model. In addition, the present invention also relates to a preparation method and application of the above-mentioned humanized glioma PDX mouse model. Background technique [0002] For a long time, in order to study tumors, it is customary to transplant tumor cell lines into immune-deficient mice to establish tumor models. However, due to the residual immune system of mice and the lack of human's own immune system, the experimental results have a large deviation from the clinical practice. Moreover, with the development of science and technology, tumor immunotherapy is another new treatment method that can improve the survival of cancer patients after surgery, radiotherapy, chemotherapy and molecular targeted therapy. Further research on tumor immunotherapy requires the establishment of animal models of the interaction between human tumors and the hum...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2207/12A01K2227/105A01K2267/0325A01K2267/0387A01K2267/0393
Inventor 刘志雄罗承科许胜超肖志强陈凌陈新建
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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