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A kind of pharmaceutical composition for preventing and/or treating cardiomyopathy and nephropathy and its preparation method and application

A composition and drug technology, applied in the field of biomedicine, to achieve the effects of improving left ventricular systolic and diastolic function, reducing myocardial fibrosis and reducing urinary protein

Active Publication Date: 2022-07-08
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Ang IV is considered to have the opposite biological effect to Ang II, the research fields of Ang IV's pharmacology, preparation and translational medicine are still rarely reported.

Method used

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  • A kind of pharmaceutical composition for preventing and/or treating cardiomyopathy and nephropathy and its preparation method and application
  • A kind of pharmaceutical composition for preventing and/or treating cardiomyopathy and nephropathy and its preparation method and application
  • A kind of pharmaceutical composition for preventing and/or treating cardiomyopathy and nephropathy and its preparation method and application

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preparation example Construction

[0046] In another specific embodiment of the present invention, there is provided a preparation method of the above-mentioned nanoparticles, the preparation method comprising:

[0047] S1, chitosan is dissolved in glacial acetic acid solution, adjust pH with saturated sodium hydroxide solution, obtain solution I;

[0048] S2, adding the Ang IV polypeptide into the sodium tripolyphosphate (TPP) aqueous solution to dissolve, adding the Ang IV polypeptide and the sodium tripolyphosphate mixed solution dropwise to the above solution I, and forming nanoparticles spontaneously by the electrostatic action of anions and cations; Wash and freeze-dry.

[0049] In another specific embodiment of the present invention, the molecular weight of chitosan is 200-2000kDa, more preferably 250-1200kDa, most preferably 600kDa, and the degree of deacetylation is greater than 90%.

[0050] In another specific embodiment of the present invention, in step S1, the pH value is adjusted to 4.5-6.0, pref...

Embodiment 1

[0062] Embodiment 1 A kind of pharmaceutical composition for treating and / or preventing cardiomyopathy and nephropathy

[0063] The pharmaceutical composition is chitosan nanoparticles encapsulating Ang IV polypeptide.

[0064] The preparation method of the chitosan nanoparticles encapsulating the Ang IV polypeptide, comprising the following steps:

[0065] (1) 30 mg of chitosan with a molecular weight of 600 kDa was dissolved in 8 mL of 10% glacial acetic acid solution, and the pH was adjusted to 5.5 with saturated sodium hydroxide solution to obtain solution I;

[0066] (2) Take 2 mL of Ang IV polypeptide solution with a concentration of 1.5 μmol / dL and 2 mL of 0.5 mg / mL sodium tripolyphosphate (TPP) aqueous solution and mix and stir (1000 rpm) evenly. Mix 1 drop of Ang IV polypeptide and TPP solution. It is added dropwise into the above solution I at a speed of / 3 to 5 seconds, and nanoparticles are formed spontaneously through the electrostatic action of anions and cation...

Embodiment 2

[0067] Embodiment 2 A kind of pharmaceutical composition for treating and / or preventing cardiomyopathy and nephropathy

[0068] The pharmaceutical composition is chitosan nanoparticles encapsulating Ang IV polypeptide.

[0069] The preparation method of the chitosan nanoparticles encapsulating the Ang IV polypeptide, comprising the following steps:

[0070] (1) 10 mg of chitosan with a molecular weight of 250 kDa was dissolved in 8 mL of 10% glacial acetic acid solution, and the pH was adjusted to 5.5 with saturated sodium hydroxide solution to obtain solution I;

[0071] (2) Take 2 mL of Ang IV polypeptide solution with a concentration of 1.5 μmol / dL and 2 mL of 0.5 mg / mL TPP aqueous solution and mix and stir (1000 rpm) evenly. Speed ​​dropwise added to the above solution I, through the electrostatic action of anions and cations to spontaneously form nanoparticles; after centrifugation, washing, freeze-drying. The suspension was centrifuged at high speed at 4°C for 20 minut...

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Abstract

The invention provides a pharmaceutical composition for preventing and / or treating cardiomyopathy and nephropathy, a preparation method and application thereof, and belongs to the technical field of biomedicine. According to the research of the present invention, Ang IV can be applied to the treatment of cardiomyopathy and nephropathy, and can be further prepared into nanoparticle pharmaceutical preparations. It has been proved by experiments that it can significantly improve the left ventricular systolic and diastolic functions of diabetic mice and reduce myocardial fibers in diabetic mice. It can reduce urinary protein, improve renal creatinine clearance rate, and at the same time, the preparation process is simple and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a pharmaceutical composition for preventing and / or treating cardiomyopathy and nephropathy, and a preparation method and application thereof. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] In recent years, due to problems such as obesity and unbalanced diet, the incidence of diabetes, hypertension, and coronary heart disease has continued to rise, and the trend of younger onset is obvious. These diseases have many complications and great harm, mainly including cardio-cerebrovascular diseases such as cardiomyopathy and cerebral infarction, as well as nephropat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61K45/06A61K9/51A61K47/36A61P9/00A61P13/12A61P3/10
CPCA61K38/085A61K45/06A61K9/5161A61K9/5192A61P9/00A61P13/12A61P3/10A61K2300/00
Inventor 郝盼盼章萌
Owner SHANDONG UNIV QILU HOSPITAL