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Use of the alkaloid compound hip in the preparation of products for preventing and/or treating heart damage

A kind of heart injury and compound technology, applied in the field of alkaloid compounds to achieve the effect of inhibiting myocardial cell toxicity

Active Publication Date: 2022-03-22
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there are still many active substances contained in seabuckthorn that have not been isolated and identified. If we can conduct more in-depth and subtle research on the new chemical components and their pharmacological effects in seabuckthorn and discuss their active mechanisms, it is expected to develop more. Safe and effective natural plant-derived medicines that can treat diseases

Method used

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  • Use of the alkaloid compound hip in the preparation of products for preventing and/or treating heart damage
  • Use of the alkaloid compound hip in the preparation of products for preventing and/or treating heart damage
  • Use of the alkaloid compound hip in the preparation of products for preventing and/or treating heart damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Inhibitory effect test of compound Hip on cardiomyocyte apoptosis in H9c2 cardiotoxicity model

[0036] 1. Toxic effect of alkaloid compound Hip on H9c2 cardiomyocytes

[0037] After H9c2 cells were incubated with different concentrations (0.1 μM, 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, 160 μM) of the alkaloid compound Hip for 24 h, the toxic effect of the alkaloid compound on cardiomyocytes was evaluated by MTT colorimetry.

[0038] 2. Effect of alkaloid compound Hip on DOX-induced toxicity of H9c2 cardiomyocytes

[0039](1) Pre-incubate H9c2 cells with alkaloid compound Hip for 1 h, then add 2.5 μM doxorubicin (DOX) and culture for 24 h, use MTT colorimetry to screen alkaloid compound Hip for DOX-induced cardiomyocyte apoptosis activity, and Determine the optimal concentration range for action.

[0040] (2) After treating the H9c2 cardiomyocytes with different seabuckthorn alkaloids for 1 hour, treat them with 2.5 μM DOX for 24 hours, then extract the prot...

Embodiment 2

[0049] Tablets containing the compound Hip

[0050] The following ingredients are produced for each tablet:

[0051]

[0052] After mixing, granulating, blending and tableting, 250mg tablets were made.

Embodiment 3

[0054] Injection containing compound Hip

[0055] Produce injections with the following components per 1 ml:

[0056]

[0057] The above solid substance was dissolved in purified water, and the solution was aseptically filled into 1 ml ampoules.

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PUM

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Abstract

The invention discloses the use of the alkaloid compound Hip in the preparation of a product for preventing and / or treating heart damage, the activity research of the alkaloid compound Hip is carried out, and it is verified that the alkaloid compound Hip provided by the invention can inhibit myocardial toxicity induced Apoptosis, by down-regulating the expression level of p-JNK and / or Cleaved-Caspase-3 to inhibit cardiomyocyte apoptosis induced by anthracycline drug DOX, thereby alleviating cardiomyocyte toxicity, and achieving prevention and / or treatment of cardiomyocyte toxicity The effect can be used to prepare medicines for preventing and / or treating diseases related to myocardial injury.

Description

technical field [0001] The invention relates to the field of medical applications of alkaloid compounds, in particular to the use of an alkaloid compound Hip in the preparation of products for preventing and / or treating heart damage. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a deciduous shrub of the family Echinaceae and the genus Hippophae. It is characterized by drought resistance, wind and sand resistance, and can survive on salinized land, so it is widely used in soil and water conservation. . Seabuckthorn is grown in large quantities in northwest China for desert greening. [0003] Seabuckthorn is rich in nutrients, including active substances such as vitamins, flavonoids, triterpenoids, oils and fatty acids, phenols, volatile oils, trace elements, phospholipids, 5-hydroxytryptamine, and various amino acids and proteins needed by the human body. [0004] Seabuckthorn seeds are often used to extract oil to make se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/437A61P9/00A61P35/00A61K45/06A61K36/185
CPCA61K31/437A61P9/00A61P35/00A61K45/06A61K36/185A61P9/04A61K9/2018A61K9/0019A61K47/26A61K9/08A61K9/2009A61K9/2013A61K9/2054A61K9/2059A61K47/02
Inventor 周文娜王洪伦欧阳健
Owner HUZHOU TEACHERS COLLEGE
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