Application of glycosyl polyether compound in preparation of paramyxovirus or enterovirus resisting medicine

A technology of polyether compound and paramyxovirus, which is applied in the field of biomedicine and can solve problems such as physical effects

Active Publication Date: 2021-06-22
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although effective vaccines have been developed against some of the entero...

Method used

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  • Application of glycosyl polyether compound in preparation of paramyxovirus or enterovirus resisting medicine
  • Application of glycosyl polyether compound in preparation of paramyxovirus or enterovirus resisting medicine
  • Application of glycosyl polyether compound in preparation of paramyxovirus or enterovirus resisting medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Select maduramycin as a representative compound of the compound shown in formula 1, and the specific structure is as follows:

[0049]

[0050] Firstly, maduramycin (Mad) was prepared into a certain concentration of mother solution with DMSO, and then diluted with DMEM medium containing 10% FBS according to the molecular weight of maduramycin to the following concentrations: 1 μM, 5 μM 2 concentration gradients, while diluting Maduramycin liposome (Mad-liposome) with DMEM medium to two concentrations of 1 μM and 5 μM.

[0051] Inoculate normal Hela cells into a 12-well plate. After about 12-16 hours, about 60% of the cells cover the bottom of the well, discard the old medium, dilute the HPIV3 virus stock solution with DMEM, and add the virus stock solution according to the multiplicity of infection moi=0.1, After 2 hours, the virus liquid was discarded, replaced with a new medium containing drugs, and co-cultivated for 24 hours. Hela cells were collected, and the ex...

Embodiment 2

[0053] Using the same method as in Example 1, the inhibitory effect of the maduramycin glycosyl polyether compound on Paramyxoviridae RSV strains was detected. Hela cells were collected, and the expression of P-glycoprotein (RSV-P) of RSV in the cells was detected by western blotting method. The experimental results were as follows: figure 2 shown. from figure 2 It can be concluded that maduramycin has a very significant inhibitory effect on Paramyxoviridae RSV strains at a concentration of 5M, and the expression of P-glycoprotein is hardly detected; the expression of P-glycoprotein is significantly weakened at a concentration of 1M. Therefore, maduramycin exhibited a strong inhibitory effect on Paramyxoviridae virus RSV at the cellular level.

Embodiment 3

[0055] Using the same method as in Example 1, the inhibitory effect of the maduramycin glycosyl polyether compound on the enterovirus EV71 strain was detected. Hela cells were collected, and the expression of the VP1 structural protein (EV71-VP1) of EV71 in the cells was detected by western blotting. The experimental results were as follows: image 3 shown. from image 3 It can be concluded that maduramycin has a very significant inhibitory effect on enterovirus EV71 strain at 5M and 1M concentrations, and the expression of VP1 structural protein is hardly seen. Therefore, maduramycin exhibited a strong inhibitory effect on Paramyxoviridae virus RSV at the cellular level.

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Abstract

The invention mainly aims to provide application of a glycosyl polyether compound as a paramyxovirus or enterovirus resisting medicine. The paramyxovirus is at least one of an HPIV3 strain and an RSV strain; and the enterovirus is at least one of an EV68 strain, an EV69 strain, an EV70 strain and an EV71 strain. A large amount of researches by the inventor prove that the glycosyl polyether compound can influence cell metabolic pathways, calcium ion channel proteins, nucleoproteins of viruses, coating precursors, glycoproteins and matrix protein functions in a large range, destroy virus replication and infection cycles, or influence functions of host cell related proteins, and can achieve a broad-spectrum antiviral effect; and particularly, the polypeptide can be used for inhibiting proliferation of paramyxovirus or enterovirus.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the use of glycosyl polyether compounds in the preparation of anti-paramyxovirus or enterovirus drugs. Background technique [0002] Paramyxoviruses are a class of single-stranded, negative-strand RNA viruses that are not segmented. The viruses that are pathogenic to humans in this family are mainly human parainfluenza virus, measles virus, mumps virus and respiratory syncytial virus. Human Parainfluenza virus type 3 (HPIV3) belongs to the Respiratory Virus genus of the Paramyxoviridae family and is one of the main pathogens causing lower respiratory tract infections in infants and immunocompromised populations. Can cause acute bronchiolitis, pneumonia and asthma. However, there are currently no effective vaccines and antiviral drugs that can effectively prevent and treat HPIV3 infection. Human respiratory syncytial virus (Human Respiratory syncytial virus, HRSV...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/14C12Q1/70C12Q1/02C12R1/93
CPCA61K31/7048A61P31/14C12Q1/025G01N2333/115G01N2333/085Y02A50/30
Inventor 刘天罡黄敏坚陈明周李智菲
Owner WUHAN UNIV
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