Primers for screening and parting enterovirus viruses and detection method

An enterovirus, enterovirus technology, applied in the field of genetic engineering, can solve the problems of false negative, high cost, insufficient sample sensitivity, etc.
CN106148570AInactive Publication Date: 2016-11-23中华人民共和国大榭出入境检验检疫局
0 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中华人民共和国大榭出入境检验检疫局
Publication Date
2016-11-23
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses primers for screening and parting enterovirus viruses and a detection method. The sequences of the primers are the CODEHOP outer primer sequence, the CODEHOP inner primer sequence and the primer sequences of 20 viruses. The detection method includes the steps of extraction of RNA; one-step RT-PCR; nest type PCR; 1.5% agarose gel electrophoresis to judge the positivity and parting result of enteroviruses. A pair of enterovirus consistent-degenerate primers is designed, a pair of short-fragment consistent-degenerate primers and parting primers of 20 different enteroviruses are designed inside amplified fragments of the primers, and in combination with the nest type RT-PCR technology, a degenerate primer nest type RT-PCR method capable of being used for screening and parting different genotype viruses of the enteroviruses is established. The advantages of being high in sensitivity and specificity and suitable for monitoring and variety identifying of enteroviruseses of low-virus-content samples are achieved.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a primer and a detection method for screening and typing Enteroviruses. Background technique

[0002] In recent years, outbreaks of human infectious diseases caused by enterovirus infection have often been reported, and have become an important public health problem; Infect humans, including poliovirus, coxsackievirus, echovirus and some new enteroviruses and hepatitis A virus. For example, in 1998, hand, foot and mouth disease and herpetic pharyngitis infected by enterovirus type 71 (EV71) broke out in Taiwan. The number of infected people exceeded 100,000. Edema and hemorrhage, acute flaccid paralysis and myocarditis died. At present, HEV accounts for about 80% to 92% of the pathogenic factors of aseptic meningitis. In recent years, some studies have shown that diabetes is also related to enteroviruses. Enteroviruses carried by patients and infected ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More