Preparation of doc2α and doc2β gene-deficient zebrafish mutants and construction method of zebrafish autism model

A zebrafish, alpha gene technology, applied in the field of genetic engineering, can solve the problems of complex experimental process, long experimental period, unsystematic research on pathogenesis, etc., and achieve the effect of simple construction method, accurate drug effect, and accurate drug toxicity.
CN113005142BActive Publication Date: 2021-11-16NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Publication Date
2021-11-16

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Abstract

The invention relates to the field of genetic technology engineering, and specifically discloses a method for preparing a doc2α and doc2β gene-deficient zebrafish mutant and a method for constructing a zebrafish autism model. The present invention utilizes CRISPR / Cas9 technology to realize double-gene-specific knockout of zebrafish doc2α gene and doc2β gene for the first time, establishes zebrafish with deletion of doc2α and doc2β genes, and forms a stable genetic zebrafish autism model. The present invention also provides a zebrafish A method for constructing a fish autism model. The constructed zebrafish autism model can be used for high-throughput drug screening more efficiently and accurately, and can more accurately evaluate drug effects and drug toxicity.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a doc2α and doc2β Preparation of gene-deficient zebrafish mutants and construction method of zebrafish autism model. Background technique

[0002] Autism Spectrum Disorder (ASD) is a developmental disorder of the central nervous system and one of the most serious neurodevelopmental disorders in children. According to the World Health Organization ICD-10 diagnostic criteria for autism, the clinical manifestations of autistic patients are mainly language barriers, social activity barriers, and limited, repetitive stereotyped behaviors, sensory system abnormalities, and some patients have obvious Specific interests, abnormal or low intelligence, about 3 / 4 of the cases have significant mental retardation and so on. Patients usually develop disease symptoms at the age of 3, and the best period of training and rehabilitation is before the age of 7. At present, many fami...

Claims

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