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Method for detecting esomeprazole by applying ultra-high performance liquid chromatography-tandem mass spectrometry

An ultra-high-efficiency liquid phase and esomeprazole technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of low sensitivity of esomeprazole detection method, achieve high sensitivity, reliable results, and research content full effect

Inactive Publication Date: 2021-06-22
江苏乾元生物科技有限公司
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Problems solved by technology

At present, the sensitivity of the detection method of esomeprazole after taking esomeprazole preparations in humans is low, which cannot provide reliable and sufficient experimental data and theoretical reference for further research on pharmacological efficacy and clinical application.

Method used

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Embodiment Construction

[0016] In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the present invention. examples, but not all examples.

[0017] The invention provides a method for detecting esomeprazole by applying ultra-high performance liquid chromatography-tandem mass spectrometry, comprising the following steps:

[0018] Step (1), take the sample to be tested, place 0.3 g of blood in a volumetric flask, add acetonitrile-water 8:2 to make the volume to 10-50 ml, vortex mixing for 5-15 minutes, ultrasonically extract for 5-15 minutes, and filter through 0.22 μm. Membrane, injection to detect esomeprazole in the sample;

[0019] In step (2), an ultra-high performance liquid chromatography-tandem mass spectro...

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PUM

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Abstract

The invention relates to a method for detecting esomeprazole by applying ultra-high performance liquid chromatography-tandem mass spectrometry, which comprises the following steps: step (1), putting a sample to be detected into a volumetric flask, adding acetonitrile and water in a ratio of 8:2 to dilute to 10-50ml, carrying out vortex mixing for 5-15 minutes, carrying out ultrasonic extraction for 5-15 minutes, passing through a 0.22 mum filter membrane, and injecting the sample to detect esomeprazole in the sample; and (2) detecting the esomeprazole by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) as a detection instrument. The detection method has the advantages of good selectivity, high sensitivity, simplicity, rapidness and the like. By using UPLC-MS / MS, the esomeprazole in bulk drugs, pharmaceutical preparations and other samples can be rapidly and efficiently subjected to limited test; according to the present invention, further optimization is performed on the basis of the existing conditions, the research content is comprehensive, the high performance liquid chromatography for esomeprazole in blood is established, and the effective analysis means is provided for the quality research and the stability investigation.

Description

technical field [0001] The invention relates to a method for detecting esomeprazole, in particular to a method for detecting esomeprazole by applying ultra-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] The chemical name of esomeprazole is S-5-methoxy-2-{[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]sulfinyl} -1H-benzimidazole, molecular formula is C 17 H 19 N 3 O 3 S, molecular weight is 345.41600, density is 1.37g / cm 3 , the boiling point is 600°C at 760mmHg, the flash point is 316.7°C, the refractive index is 1.669, and the vapor pressure is 0mmHg at 25°C. It is clinically used as an alternative therapy for gastroesophageal reflux disease when oral therapy is not suitable. At present, the sensitivity of the detection method for esomeprazole after taking esomeprazole preparations in humans is low, and it cannot provide reliable and sufficient experimental data and theoretical reference for further research on pharmacologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘长生肖石基
Owner 江苏乾元生物科技有限公司
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