Eye drop composition comprising aptamin c as active ingredient

A technology of active ingredients and compositions, applied in the field of eye drop compositions, can solve the problems of inability to obtain preservation efficacy, different physical and chemical properties, etc., and achieves improvement of epithelial cell damage, low reactive oxygen species levels, improvement and treatment of dry eye syndrome Effect

Pending Publication Date: 2021-06-25
(株)纳斯摩仕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in fact, if the concentration of preservatives in the eye drops is reduced, it will not be able to obtain sufficient preservation effect as a multi-dose type, and the change of the compounding ingredients due to t

Method used

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  • Eye drop composition comprising aptamin c as active ingredient
  • Eye drop composition comprising aptamin c as active ingredient
  • Eye drop composition comprising aptamin c as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: preparation composition of the present invention

[0031] At room temperature, mix the synthesized nucleic acid aptamer and vitamin C (DSM Company, UK) having the effect in the above-mentioned Example 1 in distilled water at the weight ratio described, thereby preparing the composition of the present invention .

[0032] For example, the eye drop composition of the present invention is prepared by mixing 0.008% (w / v) of vitamin C and 0.001% (w / v) of Aptamin C combined with the above vitamin C in distilled water.

Embodiment 2

[0033] Example 2: Maintenance of Vitamin C Reducing Power by Nucleic Aptamers

[0034] After heating Aptamin C dissolved in Annealing buffer to 95°C, slowly lower the temperature to room temperature to prepare the secondary structure of the nucleic acid aptamer, mix L-ascorbic acid and react About 30 minutes to allow the aptamer to bind to L-vitamin C. After that, hydrogen peroxide was added to create oxidation conditions, and 12-oxo-phytodienoate reductase was added as a fluorescent dye to measure the oxidation of L-vitamin C. Docosahexaenoic acid-12-oxo-plant produced by reacting docosahexaenoic acid (DHA), which is an oxide of L-vitamin C, with 12-oxo-phytodienoic acid reductase Dienoic acid reductase (DHA-OPDA) measures the amount of fluorescence to quantitatively analyze the degree of generation of docosahexaenoic acid. Under the above conditions, the fluorescence of docosahexaenoic acid-12-oxo-phytodienoic acid reductase was measured every two minutes for 12 hours. ...

Embodiment 3

[0036] Example 3: Wound Healing Experiments on Human Keratinocytes

[0037] 500 μL / well of human keratinocytes (purchased from the Institute of Life Systems, Sookmyung Women's University, Korea) were inoculated into 6-well culture plates (1×10 5 cells / ml), in 5% CO 2 , 95% air, and a temperature of 37°C using DMEM medium for culture.

[0038] The cells were wounded by scratching the surface of the culture using a 200 μL pipette.

[0039] To remove non-attached cells, the above scratched human keratinocytes were washed with fresh medium.

[0040] 1 μM (0.001%) of Aptamin C, 0.5 mM (0.008%) of vitamin C, and a mixture of Aptamin C and vitamin C were respectively treated in the culture medium and cultured for 24 hours.

[0041] Cell movement was measured using an inverted phase-contrast light microscope.

[0042]The lesion site of each well was photographed and its area was determined using Image J.

[0043] The average area between moving cell edges was determined using I...

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PUM

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Abstract

The present invention relates to an eye drop composition comprising, as active ingredients, vitamin C and an aptamer bonding to the vitamin C. As can be known from the present invention, the eye drop composition comprising aptamin C, of the present invention, enables the maintaining of a low ROS level and the improvement of epithelial cell damage and an inflammatory response, and thus, by using an aptamer and antioxidant conjugate for maintaining such antioxidant effect for a long time, an effect of improving and treating dry eye syndrome may be achieved.

Description

technical field [0001] The present invention relates to an eye drop composition comprising Aptamin C as an active ingredient. Background technique [0002] In modern society, due to wearing contact lenses, pressure from technology, contact with allergens, air pollution and other reasons, the pressure on the cornea or conjunctiva is gradually increasing. Therefore, people suffering from dry eye syndrome (dry eye) The number of people is also increasing. If you suffer from dry eye, the normal function of the cornea or conjunctiva will be reduced, and it is easy to cause corneal epithelial disorder, corneal epithelial erosion, corneal ulcer, conjunctivitis, keratitis, eye infection, etc. [0003] In the past, the well-known method of preventing or treating dry eye or eye diseases accompanied by corneal or conjunctival dysfunction is as follows: by instilling eye drops containing viscoelastic substances such as chondroitin sulfate and hyaluronic acid to the eyeball to protect t...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K9/08A61K31/375A61K31/7088A61P27/00A61P27/04
CPCA61P27/00A61P27/04A61K9/08A61K31/375A61K31/711A61K9/0048A61K2300/00
Inventor 孙寅植
Owner (株)纳斯摩仕
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