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Preparation technology and use of suitable microecological microbial agent for individually solving continuous cropping obstacles of strawberries

A micro-ecological bacterial agent and continuous cropping obstacle technology, which is applied in the field of personalized customization of micro-ecological organic fertilizers, can solve the problems of untargeted and single-function microbial fertilizers, and achieve the effects of inhibiting growth and reproduction and delaying exhaustion

Inactive Publication Date: 2021-07-02
银川尧玥生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at problems such as the single function and lack of pertinence of the existing microbial fertilizers, the present invention provides the preparation technology and use of micro-ecological bacteria agents that can solve strawberry continuous cropping obstacles in a personalized way, and determine the unique microbial bacteria according to the distribution of strawberry soil microorganisms in different regions. Fermentation of natural plants to obtain suitable microbial fertilizers. The formula of microbial agents is formulated according to the nature of the soil. The development of microbial fertilizers for strawberry crops is targeted and unique.

Method used

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  • Preparation technology and use of suitable microecological microbial agent for individually solving continuous cropping obstacles of strawberries
  • Preparation technology and use of suitable microecological microbial agent for individually solving continuous cropping obstacles of strawberries
  • Preparation technology and use of suitable microecological microbial agent for individually solving continuous cropping obstacles of strawberries

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Embodiment 1

[0036] A customer of Helan, Yinchuan, Ningxia provided strawberry soil samples and needed to customize bio-fertilizers that meet the needs of improving soil obstacles to continuous cropping of strawberries. Firstly, the strawberry soil microbial database in Yinchuan Helan area was constructed, and the microbial composition distribution ratio information in 300 strawberry soil samples in the area was obtained through high-throughput 16SrRNA gene sequencing. Ecological agent formula:

[0037] (1) Treatment of strawberry soil samples: microbead crushing method was used to extract and purify the microbial DNA in the sample, and 515F / 806R PCR primers were used to amplify the V4 hypervariable region of the 16S rRNA gene of the microbial DNA; 515F / 806R PCR Primers were 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0038] (2) Determination of the total amount of microorganisms in the soil: extract the total DNA of microorganisms in the soil, then use 16S rRNA as the target...

Embodiment 2

[0046] Customers in the Xixia region of Ningxia provided strawberry soil samples and needed to customize biofertilizers that meet their soil needs. Firstly, build a soil microbial database in the Xixia region of Ningxia, obtain the distribution ratio information of microbial composition in 200 strawberry soil samples in the region through high-throughput 16S rRNA gene sequencing, and then select some strains according to the test results to formulate a suitable microecological Bacteria formula:

[0047] (1) Soil sample treatment: Microbead crushing method was used to extract and purify the microbial DNA in the sample, and 515F / 806R PCR primers were used to amplify the V3-V4 hypervariable region of the 16S rRNA gene of the microbial DNA; 515F / 806R PCR primers were 515F: GTGCCAGCMGCCGCGGTAA, 806R: GGACTACHVGGGTWTCTAAT.

[0048] (2) Determination of the total amount of microorganisms in the soil: extract the total DNA of microorganisms in the soil, then use 16S rRNA as the targe...

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Abstract

The invention discloses a preparation technology and use of a suitable microecological microbial agent for individually solving continuous cropping obstacles of strawberries, and belongs to the technical field of personalized customization of microecological organic fertilizers. The preparation technology is mainly characterized by performing sampling and sequencing analysis on continuous cropping soil of strawberries, and customizing the formulation of microbial agents for the specific soil through compound microbial florae (mainly including photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria, bacillus subtilis, bacillus mucilaginosus, trichoderma, aspergillus niger, actinomycetes, nitrogen-fixing bacteria, beneficial gram positive bacteria, cellulose decomposition bacteria and the like); and using natural plants as a fermentation substrate for fermentation, and obtaining high-quality microbial dynamic inoculant after sufficient decomposition, so that continuous cropping obstacles of strawberries are improved, the stability of strawberry products is enhanced, the quality and yield of the strawberry products are improved, and the problem of a traditional single production process is solved. The method is fully and accurately applied in the field of modern agriculture, and fills the industrial blank.

Description

technical field [0001] The invention belongs to the technical field of individualized customization of micro-ecological organic fertilizers. Sampling and sequencing analysis are carried out for the continuous cropping soil of strawberries, and the specific soil is customized and prepared according to different microbial flora. Comply with the preparation technology and use of microecological bacteria agent. Background technique [0002] In the strawberry production mode, due to the high cost of greenhouse construction, it takes 9 months for the growth cycle of strawberries to mature and harvest from a fixed value, so it is difficult to rotate crops during production, and continuous cropping obstacles are serious. Greenhouses that grow strawberries all year round will suffer from pests and diseases Serious, unbalanced soil pH, and some soil-borne diseases occur year after year, resulting in a decline in the quality and yield of strawberry production in the later stages of str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/80C05G5/20C05F17/20C05F17/50A01C21/00
CPCC05F11/00C05F17/00A01C21/007Y02W30/40
Inventor 尹星徐新燕吴欣圆任淑媛孙子茜
Owner 银川尧玥生物科技有限公司
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