Pregabalin artificial hapten, artificial antigen and preparation method and application thereof

A technology of artificial hapten and pregabalin, which is applied in the field of pregabalin artificial hapten, artificial antigen and its preparation, can solve the problems of time-consuming detection, expensive equipment, failure to meet the requirements of fast and accurate, and achieve high affinity, Sensitive and accurate immunoassays and effects of immunoassays

Active Publication Date: 2021-07-09
HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the detection of pregabalin mainly relies on high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), mass spectrometry (MS), etc., but there are expensive instruments, time-consuming detection, and Professional and technical personnel are required to operate, and it cannot meet the fast and accurate requirements of modern detection

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  • Pregabalin artificial hapten, artificial antigen and preparation method and application thereof
  • Pregabalin artificial hapten, artificial antigen and preparation method and application thereof
  • Pregabalin artificial hapten, artificial antigen and preparation method and application thereof

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Embodiment 1

[0053] The preparation method of the embodiment of the present invention pregabalin artificial antigen (reaction process is as follows figure 1 ), including the following steps:

[0054] (1) Preparation of artificial hapten:

[0055] ① Weigh 200mg (1.258mmol) of pregabalin and place it in a 50ml single-necked round bottom flask, add 10ml of methanol and 0.5ml of concentrated sulfuric acid, add a stirrer, and place it in an oil bath at 68°C for reflux reaction for 24 hours; after the reaction, Cool to room temperature, slowly add NaHCO to the reaction system 3 The solid was adjusted to pH=7-8, and the residue was evaporated to dryness under reduced pressure, extracted with 15 ml of tetrahydrofuran, filtered, and dried to obtain 210 mg (1.214 mmol) of slightly yellow oily product A;

[0056] ②In a 50ml single-necked round bottom flask, dissolve 210mg (1.214mmol) of the slightly yellow oily product A in the previous step in 10ml of dichloromethane, add 238mg (2.428mmol) of male...

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Abstract

The invention discloses a pregabalin artificial hapten, an artificial antigen as well as a preparation method and application of the pregabalin artificial hapten and the artificial antigen. The molecular structural formula of the pregabalin artificial hapten is as shown in formula (I), and the molecular structural formula of the pregabalin artificial antigen is as shown in formula (II). The application refers to the application of the pregabalin artificial antigen in preparation of an anti-pregabalin antibody. The pregabalin artificial hapten maximally retains the characteristic structure of pregabalin, has an active group capable of being coupled with carrier protein, and can be used as an antigenic determinant; the further prepared pregabalin artificial antigen can be immunized to obtain an anti-pregabalin antibody with high affinity, high sensitivity and strong specificity, the titer of immune serum obtained by immunizing a New Zealand white rabbit reaches up to 1: 90000, and the pregabalin artificial antigen can be used for rapid and accurate immunodetection and immunoassay of pregabalin.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a pregabalin artificial hapten, artificial antigen and a preparation method and application thereof. Background technique [0002] As an anticonvulsant, pregabalin is mainly used in the treatment of partial onset epilepsy, diabetic neuralgia, postherpetic neuralgia and anxiety disorders in adults. It is a GABA analog that reduces the release of neurotransmitters such as glutamate, norepinephrine, substance P, and calcitonin gene-related peptide. Recent studies have found that it may also play a role in the treatment of addiction to tobacco, alcohol, and benzodiazepines. [0003] Although reports of pregabalin abuse are very rare, attention should be paid especially to those patients who are prone to substance dependence. If you take more than the recommended dose of pregabalin or use the wrong way of administration (such as rectal or intranasal admin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C233/31C07C231/02C07K14/765C07K16/44G01N33/53
CPCC07C233/31C07K14/765C07K16/44G01N33/948G01N33/9473C07C231/14C07C235/76
Inventor 邵越水王镇
Owner HANGZHOU TONGZHOU BIOTECHNOLOGY CO LTD
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