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Novel amphiphilic protein as well as preparation method and application thereof

An amphiphilic, protein-based technology, applied in the fields of peptide/protein components, chemical instruments and methods, animal/human proteins, etc., can solve problems such as loss of insulin activity, improve stability, reduce medical costs, and improve the treatment of pain. Effect

Active Publication Date: 2021-07-16
李瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical feature discussed in this patented technology relates to improving the stable and long-lasting delivery (oral) medicine called Oxyntaz®, allowing it to effectively treat type 2 diarrhea without causing side effects such as weight gain due to increased blood glucose levels. Additionally, making sure certain types of medications are taken properly reduces their overall health risks associated with these conditions.

Problems solved by technology

This patents describes how biologically active compounds can become more effective at treating certain medical conditions because they may only work when needed without being affected negatively. There has been an increasing number of studies showing that these agents were able to break down quickly inside tissues called blood vessels, which makes them difficult to administer effectively outside body environments where other methods had previously worked well. One solution proposed was making it easier to make sure there wasn't any harmful residues left behind after drug administration.

Method used

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  • Novel amphiphilic protein as well as preparation method and application thereof
  • Novel amphiphilic protein as well as preparation method and application thereof
  • Novel amphiphilic protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Fermentation Expression of Two-Pentathic Protein 1

[0072] According to the existing method, plasmid construction and fermentation expression were performed on the two-proprin 1. The plasmid was transformed into E. coli and was first enlarged by graded fermentation process, and IPTG induced in the range of 45-60. After the induction, the oxygen was controlled by 20% -40%, and the medium residue 95%. The product is confirmed by SDS-Page for molecular weight (specification figure 1 The arrow refers to the two-proprime protein 1).

Embodiment 2

[0073] Example 2 Preparation, purity of the two-proprin 2

[0074] Plasmid construction and fermentation expression were performed on the two-parent protein 2 in the industry's general method. The plasmid was transformed into E. coli and was first enlarged by graded fermentation process, and IPTG induced in the range of 45-60. After the induction, the oxygen was controlled by 20% -40%, and the medium residue 95%. The product uses HPLC to make purity confirmation (specification figure 2 The HPLC map showed that the protein purity was 96.8%).

Embodiment 3

[0075] Example 3 Preparation of Two-Feelbent Protein 1 and Insulin Composition (Molar 1: 25)

[0076] 2.0 mg of two-proprin 1 lyophilized powder, dissolved in phosphate buffer 1 ml pH 5.8, then weighted 80.0 mg of insulin-free dried powder, and added to the solution of the above-mentioned amphiphilic protein, sufficiently mixing 6 After hours, the freeze dried to give the composition solid powder.

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Abstract

The invention provides an amphiphilic protein, a preparation method thereof, a carrier based on the amphiphilic protein, a pharmaceutical composition prepared from the carrier and application thereof. The carrier provided by the invention can be used as an oral administration carrier or a long-acting administration carrier of insulin, and is used for preparing a medicine for treating diabetes mellitus.

Description

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Claims

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Application Information

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Owner 李瑛
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