Receptor reagent and application thereof
A reagent and receptor technology, applied in the field of receptor reagents, can solve problems such as extremely high sensitivity requirements, and achieve the effect of meeting the sensitivity requirements, strong anti-interference ability of samples, and good test performance.
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[0124] In some embodiments of the present invention, the metal chelate comprises a chelating agent selected from the group consisting of: 4'-(10-methyl-9-anthracenyl)-2,2':6'2"-linked Tripyridine-6,6"-dimethylamine]tetraacetic acid (MTTA), 2-(1',1',2',2',3',3'-heptafluoro-4',6'-hexanedi Keto-6'-yl)-naphthalene (NHA), 4,4'-bis(2",3",3"-heptafluoro-4",6"-hexanedione-6"-yl)-o- Terphenyl (BHHT), 4,4'-bis(1”,1”,1”,2”,2”,3”,3”-heptafluoro-4”,6”-hexanedione-6”- base)-chlorosulfo-o-terphenyl (BHHCT), 4,7-biphenyl-1,10-phenanthroline (DPP), 1,1,1-trifluoroacetone (TTA), 3-naphthalene Acyl-1,1,1-trifluoroacetone (NPPTA), naphthyltrifluorobutanedione (NTA), trioctylphosphine oxide (TOPO), triphenylphosphine oxide (TPPO), 3-benzoyl- 1,1,1-trifluoroacetone (BFTA), 2,2-dimethyl-4-perfluorobutyryl-3-butanone (fod), 2,2'-bipyridyl (bpy), bipyridyl Carboxylic acids, azacrown ethers, azacryptands and trioctylphosphine oxide and their derivatives. In some embodiments of the present invention...
Embodiment 1
[0143] The preparation of embodiment 1 acceptor reagent A
[0144] 1.1 Preparation and characterization of aldehyde-based polystyrene latex microspheres
[0145] 1) Prepare a 100ml three-neck flask, add 40mmol styrene, 5mmol acrolein, and 10ml water, stir for 10min, and pass N 230min;
[0146] 2) Weigh 0.11g of ammonium persulfate and 0.2g of sodium chloride, dissolve them in 40ml of water to form an aqueous solution. This aqueous solution is joined in the reaction system of step 1), continues logical N 2 30min;
[0147] 3) The temperature of the reaction system was raised to 70° C., and the reaction was carried out for 15 hours;
[0148] 4) Cool the emulsion after the reaction to room temperature, and filter with a suitable filter cloth. The obtained emulsion is washed with deionized water for several times by centrifugal sedimentation until the conductivity of the supernatant at the beginning of centrifugation is close to that of deionized water, then diluted with wate...
Embodiment 2
[0170] The mensuration of the zeta potential of acceptor particle in the acceptor reagent of embodiment 2
[0171] The present invention adopts the detection method of ZETA potential: NICOMP 380Z3000 uses Doppler electrophoresis method (ELS) to measure the ZETA potential value. Mainly by measuring the electrophoretic migration rate of charged particles in suspension, the zeta potential value is obtained. The stability of the colloid was judged by measuring the zeta potential of the microsphere. The main factor affecting the zeta potential of particles is the charge on the particle surface.
[0172] The mensuration method of zeta potential of the present invention is:
[0173] 2.1. Prepare different acceptor reagents according to the method described in Example 1, wherein in each acceptor reagent, the sugar content per mg of the acceptor particles detected by the anthrone method is greater than 40 micrograms, as shown in Table 1.
[0174] 2.2. To prepare samples, dilute the ...
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