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Method and kit for rapidly identifying Rongchang pork

A kit and pork technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of detection technology methods for Rongchang pork authenticity identification, lack of technical support for market supervision and management, etc., and achieve convenient operation. Fast and accurate results

Pending Publication Date: 2021-07-23
CHONGQING ACAD OF ANIMAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, there has been a lack of detection techniques and methods for identifying the authenticity of Rongchang pork on the market, and the market supervision and management lacks the necessary technical support

Method used

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  • Method and kit for rapidly identifying Rongchang pork
  • Method and kit for rapidly identifying Rongchang pork
  • Method and kit for rapidly identifying Rongchang pork

Examples

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Embodiment 1

[0024] 1. The principle of competitive PCR reaction amplification

[0025] The principle of competitive PCR amplification is as follows figure 1 As shown, the whole genome sequence of Rongchang pig on NCBI was analyzed, and it was found that the miRNA-182 gene sequence (SEQ ID NO.1) of Rongchang pig has a high frequency of G / A mutation, and this locus is relatively highly conserved in other pig species . Therefore, a universal upstream primer and a competitive downstream outer primer were designed for the upstream and downstream sequences of the miRNA-182 gene in Rongchang pigs. The downstream outer primer artificially introduced a mismatch A at the -3 position at the 3' end. A competitive downstream inner primer was designed for the high-frequency mutation site G→A of the Rongchang pig miRNA-182 gene. The 3' end T of the inner primer was complementary to the mutation site A, and the 3' end of the inner primer was at the -2 site. A mismatch A is artificially introduced.

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Abstract

The invention belongs to the technical field of molecular biology application, and particularly relates to a method and a kit for rapidly identifying Rongchang pork. According to the method, high-frequency G / A mutation on the miRNA-182 gene of the Rongchang pork is detected through competitive PCR amplification, and the obtained products are two fragments with different sizes, so that the authenticity of the Rongchang pork can be identified. The identification method is an efficient and specific method for identifying the authenticity of the Rongchang pork, can be used for accurately identifying counterfeit Rongchang pork or pork products which are counterfeit by using other animal-derived components such as duck, chicken and the like or soybean components with convenient, quid and accurate operation, and is convenient for grass-root supervision departments and enterprises to rapidly identify the counterfeit brand pork.

Description

technical field [0001] The invention belongs to the technical field of molecular biology application, and in particular relates to a method and a kit for rapidly identifying Rongchang pork. Background technique [0002] Rongchang pig is one of the three best local breeds in my country and has been listed as a national key protected breed of livestock and poultry. Rongchang pork has good quality and high commercial value, and is very popular among consumers. Due to the relatively large fluctuations in domestic pork prices, especially the rising prices of high-quality pork made from local pigs, the phenomenon of pork adulteration in the market has also emerged one after another. Some unscrupulous traders pretend to be pork belly with cheap duck meat. Some unscrupulous merchants use soybean flour to add a lot of essence to make "meat powder floss", and sell it as meat floss made of pure pork. It is not uncommon to use ordinary pork to pretend to be high-quality brand pork. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2537/161
Inventor 布丽君李星解华东李睿巍薇张晓春景绍红梅学华欧秀琼
Owner CHONGQING ACAD OF ANIMAL SCI
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