Pulmonary tuberculosis variation activity marker, kit, method and model construction method

A pulmonary tuberculosis and active technology, applied in the field of biomedicine, can solve the problems of low sensitivity and poor specificity, and achieve the effect of overcoming low detection rate, good specificity and good clinical application value.

Pending Publication Date: 2021-07-27
SHANGHAI PUBLIC HEALTH CLINICAL CENT
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses two different proteins from MIP-3 B lymphocyte stimulation factor bacteria called Plasmodium immune complexes PICO 19a and Toll-like receptor 4 LTR4/5 that can detect changes caused by certain substances like antibiotics or other drugs used during treatment on people with lung cancer. These markers are quickly detected but they may also be affected due to factors such as inflammatory responses associated with chemotherapy treatments. Therefore this method offers potential diagnoses based solely upon these indicators without affecting any others' healthy cells.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding new ways to diagnosing lung cancer without relying on expensive or invasively collected samples like blood testing due to its low sensitivity and potential false positives.

Method used

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  • Pulmonary tuberculosis variation activity marker, kit, method and model construction method
  • Pulmonary tuberculosis variation activity marker, kit, method and model construction method
  • Pulmonary tuberculosis variation activity marker, kit, method and model construction method

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specific Embodiment approach

[0055] The present invention will be further described below in conjunction with specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

[0056] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

Embodiment 1

[0058] Isolation of plasma samples from whole blood

[0059] The fasting peripheral blood of patients with active pulmonary tuberculosis was collected in the morning with EDTA anticoagulant tubes, centrifuged at 3000 rcf for 15 min, and the plasma was separated into a new 1.5 mL centrifuge tube within 6 hours. Plasma samples were stored in a -80°C freezer.

Embodiment 2

[0061] Determination of Protein Expression Level in Plasma Samples by ELISA

[0062] Plasma sample collection: 88 cases of active tuberculosis patients, 88 cases of healthy controls, and 88 cases of non-tuberculosis pulmonary respiratory diseases.

[0063] The expression levels of plasma protein markers were detected using human C1QB and CCL19 (MIP3B) kits (CLOUD-CLONE CORP.Whan.CN) according to standard operations. The t test or one-way ANOVA in GraphPad Prism 8 software was used for statistical analysis (when Pfigure 1 shown.

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Abstract

The invention relates to a pulmonary tuberculosis variation activity marker, a kit, a method and a model construction method, and the pulmonary tuberculosis variation activity marker is characterized in that the pulmonary tuberculosis variation activity marker is a plasma protein biomarker in a blood sample, and the plasma protein biomarker is a combined marker comprising one or more of C1QB protein and CCL19 (MIP3B) protein; a combined marker of C1QB and CCL19 (MIP3B) is used as a biomarker for detecting the activity of pulmonary tuberculosis for the first time, a rapid diagnosis model for detecting the activity of pulmonary tuberculosis is constructed, a new direction is provided for clinical diagnosis of tuberculosis, and as a technical conception for diagnosing the activity of pulmonary tuberculosis, the biomarker provides a new target spot for the diagnosis of the active tuberculosis; therefore, the invention overcomes the defects of low detection rate, long time consumption and the like of the existing active tuberculosis diagnosis, has the characteristics of good specificity and high sensitivity, and has good clinical application value for auxiliary diagnosis of tuberculosis activity.

Description

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Claims

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Application Information

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Owner SHANGHAI PUBLIC HEALTH CLINICAL CENT
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