Using method and manufacturing method of spore germination mechanism research device based on dielectrophoresis
A technology of dielectrophoresis and a manufacturing method, which is applied in the field of spore germination mechanism research devices based on dielectrophoresis, can solve the problems of inability to understand the situation of spore germination, inability to study the communication mechanism principle of spore germination, inability to determine chemical substances and other problems
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Embodiment 1
[0041] Such as Figure 1-3 As shown, a device for studying the mechanism of spore germination based on dielectrophoresis includes a microchannel 6, a first electrode 31 and a second electrode 34, and the first electrode 31 and the second electrode 34 are relatively spaced to form the microchannel. Channel 6 ; one end of the gap between the first electrode 31 and the second electrode 34 serves as the inlet 61 of the microchannel 6 , and the other end serves as the outlet 62 of the microchannel 6 .
[0042] In this spore germination mechanism research device, the first electrode 31 and the second electrode 34 constitute the two poles of the electrophoretic electric field, the gap between the first electrode 31 and the second electrode 34 forms the microchannel 6, and the two ends of the gap form the microchannel 6 respectively. The inlet 61 and the outlet 62 of the microchannel 6, when the first electrode 31 and the second electrode 34 are respectively connected to the AC power ...
Embodiment 2
[0070] Such as Figure 10 Shown, the using method of above-mentioned spore germination mechanism research device, comprises the steps:
[0071] Step 1: providing the above-mentioned device for studying the mechanism of spore germination;
[0072] Step 2: Pass the spore suspension into the microchannel 6 of the spore germination mechanism research device through the inlet 61, and connect the first electrode 31 and the second electrode 32 of the spore germination mechanism research device. Change the power supply to generate an electrophoretic electric field of a specific alternating frequency in the microchannel 6, and then make the spores in the spore suspension in the microchannel 6 undergo dielectrophoresis and attach to and fix on the microchannel 6. on both sides of the surface;
[0073] Step 3: The germination agent is constantly passed into the microchannel 6 through the inlet 61 to induce the germination of the spores attached to and fixed on the surfaces of both side...
Embodiment 3
[0076] Such as Figure 11 Shown, the manufacturing method of above-mentioned spore germination mechanism research device, comprises the steps:
[0077] Step 1: Covering a layer of insulating material on the base substrate 1 to form the first insulating layer 2;
[0078] Step 2: Covering a layer of electrode material on the first insulating layer 2 to form the electrode layer 3;
[0079] Step 3: Etching the electrode layer 3 to form a first electrode 31 and a second electrode 32, the first electrode 31 and the second electrode 32 are arranged at intervals to form a microchannel 6; the first electrode 31 and the gap one end of the second electrode 32 as the inlet 61 of the microchannel 6, and the other end as the outlet 62 of the microchannel 6;
[0080] Step 4: making a covering layer of insulating material on the etched electrode layer 3 and in the microchannel 6 to form the second insulating layer 4;
[0081]Step 5: Etching the first insulating layer 2 and the second insul...
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