Freeze-drying protective agent for prolonging normal-temperature preservation of probiotics and freeze-drying method
A technology of freeze-drying protective agent and normal temperature storage, which is applied to the preservation of microorganisms, etc., and can solve the problems of heavy workload, cumbersome methods, and high cost
Examples
Embodiment 1
[0036] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:
[0037] Step 1: Take raw materials in parts by weight, including: 17 parts of trehalose, 5 parts of linolenic acid, 10 parts of mannitol, 6 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1 part, 2 parts of sodium citrate and 10 parts of lentinan;
[0038] Step 2: Add 1 part of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;
[0039] Step 3: Place the freeze-dried reagents under the condition of vacuum degree of 0.01 mbar, respectively, at the temperature of -20°C for 2 hours, at the temperature of 15°C for 10 hours, and at the temperature of 10°C for 1 hour.
Embodiment 2
[0041] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:
[0042] Step 1: Take raw materials in parts by weight, including: 18 parts of trehalose, 6 parts of linolenic acid, 10 parts of mannitol, 7 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1 part, 3 parts of sodium citrate and 11 parts of lentinan;
[0043] Step 2: Add 1 to 3 parts of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;
[0044] Step 3: The lyophilized reagents were placed in a vacuum of 0.01 mbarr, at a temperature of -20°C for 2.5 hours, at a temperature of 18°C for 15 hours, and at a temperature of 10°C for 1.2 hours.
Embodiment 3
[0046] A kind of freeze-drying method of the freeze-drying protectant that prolongs probiotic normal temperature preservation, comprises the steps:
[0047] Step 1: Take raw materials in parts by weight, including: 19 parts of trehalose, 10 parts of linolenic acid, 10.5 parts of mannitol, 7 parts of serum albumin, 0.01 part of surfactant, 0.01 part of defoamer, sodium chloride 1.2 parts, 3 parts of sodium citrate and 12 parts of lentinan;
[0048] Step 2: Add 1 to 3 parts of glucoamylase and probiotic bacteria liquid to the mixture in step 1 to make a freeze-dried reagent;
[0049] Step 3: Place the freeze-dried reagents at a vacuum of 0.01 mbar and at a temperature of -20°C for 2.5 hours, at a temperature of 20°C for 1.5 hours, and at a temperature of 10°C for 1.5 hours.
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Description
Claims
Application Information
- IPC
- C12N1/04
- CPC
- C12N1/04
- Inventors
- 周英; 刘帅