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Gene chips, molecular probe combinations, kits and applications for analyzing sheep milk production performance

A molecular probe and gene chip technology, applied in the field of sheep milk production performance SNP site combination, can solve the problems of insufficient site uniformity, insufficient functional sites and regional representation, etc., to shorten the generation interval and save breeding costs. , Universal effect

Active Publication Date: 2021-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sheep SNP chip is mainly based on the data of western sheep breeds, lacks the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, and has insufficient locus uniformity, insufficient reflection of functional loci and regions, and more than 10% There are many problems such as extremely low frequency loci in Chinese sheep populations. It is of great significance to design a SNP chip suitable for Chinese sheep populations that can quickly and effectively detect milk production performance.

Method used

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  • Gene chips, molecular probe combinations, kits and applications for analyzing sheep milk production performance
  • Gene chips, molecular probe combinations, kits and applications for analyzing sheep milk production performance
  • Gene chips, molecular probe combinations, kits and applications for analyzing sheep milk production performance

Examples

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Effect test

Embodiment 1

[0047] Example 1 Obtaining the combination of SNP loci for milk production performance traits

[0048] 1. Selection of individual sheep

[0049] In order to achieve a more comprehensive coverage of domestic and foreign sheep breeds, the applicant collected genetic information from 248 sheep individuals in Asia, Europe, Africa and the Middle East, including 16 Asian mouflon breeds and 172 local breeds And 60 breeds, specifically related to small-tailed Han sheep, Surabaya fur sheep, big-tailed Han sheep, Wadi sheep in Shandong, China, Hu sheep in Jiangsu, China, Tan sheep in Ningxia, China, Altay sheep and Bashbai sheep in Xinjiang, China , Dorper sheep, Chinese Merino sheep (fine-wool sheep), Chinese Merino sheep (super-fine wool sheep), Suffolk sheep, Celler black sheep, Duolang sheep, Waggir Sheep sheep, Finland's Finnish sheep ( Finnsheep), Dutch Ouessant, Shetland, Solognote, Gotland, Drente Heathen, Bonga Sheep, Afar Sheep in Ethiopia, Mbororo Sheep in Niger, Yankasa Sh...

Embodiment 2

[0062] Example 2 Analyzing the Primer Combination and Probe Combination of Sheep Milk Production Performance

[0063] Those skilled in the art design primers according to the sequence information of each site in the milk production performance SNP site combination provided by the present invention, and use the designed primers for secondary structure evaluation and Tm value evaluation, and finally obtain specificity. , High sensitivity, and primers that can achieve detection purposes under the same reaction conditions.

[0064] Wherein, the evaluation of secondary structure and Tm value can be carried out by any method commonly used in this field, for example, using DNA folding form to evaluate its secondary structure, see (http: / / unafold.ma.albany.edu / ? q=mfold / DNA-Folding-Form), and then use the software RaW-Probe to evaluate its Tm value.

[0065] The above methods are conventional methods. According to the site information in the milk production performance SNP site combi...

Embodiment 3

[0067] Example 3 Analysis of the gene chip for milk production performance of sheep

[0068] The SNP gene chip of the present application adopts conventional method to immobilize the primers or probes obtained in Example 2 on polymer substrates, such as nylon membranes, nitrocellulose membranes, plastics, silica gel wafers, micro-magnetic beads, etc., or to immobilize the probes The primers or probes obtained in Example 2 are directly synthesized on a glass plate, or on a hard surface such as glass, and the use method of the SNP gene chip of the present application is the same as the conventional method.

[0069] It should be noted that those skilled in the art can use any method to prepare the SNP gene chip for detecting the milk production performance of sheep, and can also entrust a biological company to prepare it, but the SNP site combination prepared based on the milk production performance provided by this application All SNP gene chips belong to the protection scope of...

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Abstract

The invention discloses a gene chip, molecular probe combination, kit and application for analyzing the milk production performance of sheep, and relates to the field of biotechnology. The invention provides a combination of 2030 SNP sites capable of analyzing the milk production performance characteristics of sheep, and its physical location The information is determined based on the comparison of the sheep v4.0 genome sequence. Using 2030 SNP site combinations and molecular probe combinations, gene chips, and kits formed based on the site combinations can carry out genetic evaluation of sheep individuals, and it is difficult to measure in the early stage. Milk production traits are selected individually, the breeding process is controlled, and breeding costs are saved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of biodetection technology, and more specifically to the combination of SNP sites for milk production performance of sheep and its application. Background technique [0002] Multiparous sheep usually cannot secrete enough milk to meet the growth and development needs of lambs, resulting in a decrease in the survival rate of lambs. Although the use of milk substitutes can alleviate the plight of insufficient milk production in ewes to a certain extent, the fundamental solution to the problem is still Increase milk production of ewes. In addition, compared with goat's milk and cow's milk, sheep's milk has higher total fat, milk protein and lactose content, and the nutritional content of goat's milk is close to that of human's milk. One of the sources of food. However, among the 158 million sheep in our country, the number of milk sheep is almost zero. Therefore, the developm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner CHINA AGRI UNIV