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Test method for ulcerative colitis and primary sclerosing cholangitis

An inspection method, a technology for colitis, applied in chemical instruments and methods, digestive system, pharmaceutical formulations, etc., can solve problems such as unclear causes

Pending Publication Date: 2021-08-20
KYOTO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Primary sclerosing cholangitis is considered a multifactorial disease that includes immune abnormalities, but the cause is unknown

Method used

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  • Test method for ulcerative colitis and primary sclerosing cholangitis
  • Test method for ulcerative colitis and primary sclerosing cholangitis
  • Test method for ulcerative colitis and primary sclerosing cholangitis

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Experimental program
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Embodiment

[0292]

[0293] In order to identify the antigens of autoantibodies specific to patients with ulcerative colitis, the present inventors immobilized the extracellular matrix proteins known in the literature as antigen candidates on solid phases respectively, and confirmed the autoantibodies in ulcerative colitis by ELISA (Enzyme-Linked ImmunoSorbent Assay) method. Whether the sera of patients with colitis contain antibodies that bind to the extracellular matrix protein.

[0294] In the ELISA method, human IgG that can be contained in the serum of patients with ulcerative colitis and binds to an antigen candidate immobilized on a solid phase is used as an autoantibody to be detected, and rabbits labeled with HRP (horseradish peroxidase) Anti-human IgG polyclonal antibody was used as the detection antibody.

[0295] 1. Antigen candidate

[0296] As antigen candidates, the following proteins known as extracellular matrix proteins were used.

[0297] Human Integrin α2bβ3 (7148-...

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Abstract

The purpose of the present invention is to provide a test method and the like that is specific for ulcerative colitis and for primary sclerosing cholangitis. The present invention firstly provides a test method for ulcerative colitis, the test method being a detection step of detecting, in a sample and as an indicator of ulcerative colitis, an antibody that immunologically reacts with integrin [alpha]V[beta]6 and / or an antibody that immunologically reacts with integrin [alpha]V[beta]3. The present invention secondly provides a test method for primary sclerosing cholangitis, the test method being a detection step of detecting, in a sample and as an indicator of primary sclerosing cholangitis, an antibody that immunologically reacts with an antibody that immunologically reacts with integrin [alpha]V[beta]6 and / or integrin [alpha]V[beta]3.

Description

technical field [0001] One aspect of the present invention relates to an examination method for ulcerative colitis (UC) and an examination kit for examining UC. [0002] Another aspect of the present invention relates to a method for examining primary sclerosing cholangitis (PSC) and an examination kit for examining PSC. Background technique [0003] In ulcerative colitis, erosion or inflammatory ulcers of the large intestine mucosa occur continuously from the rectum, accompanied by symptoms such as diarrhea, bloody stool, and abdominal pain. Ulcerative colitis mainly occurs in the young and even the elderly, and because of its repeated active and remission periods, long-term treatment is necessary. In recent years, ulcerative colitis has been increasing worldwide. [0004] Primary sclerosing cholangitis (PSC) is a progressive chronic liver disease in which multiple and diffuse strictures occur in extrahepatic and extrahepatic bile ducts. Primary sclerosing cholangitis is...

Claims

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Application Information

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IPC IPC(8): G01N33/53C07K14/705
CPCC07K14/705G01N2333/70546G01N2800/065G01N33/6893A61P1/04A61K31/453A61K31/52A61K39/3955A61K45/06G01N33/5008G01N33/6857
Inventor 塩川雅広桑田威児玉裕三妹尾浩千叶勉
Owner KYOTO UNIV