A mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles

The technology of glomus fungus and mycorrhizal fungus is applied in the field of agricultural microorganisms to achieve the effects of fast root growth, improved cultivation efficiency and alleviation of obstacles to continuous cropping of apples.

Active Publication Date: 2022-04-15
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no related reports on the use of mycorrhizal bacteria to cultivate rootstocks resistant to apple cropping obstacles

Method used

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  • A mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles
  • A mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles
  • A mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening of efficient dominant strains

[0035] Collect soil samples from apple rhizosphere in Wantou Village, Shahe Town, Laizhou, quickly freeze and bring them back, and send them to Meiji Biotech for high-throughput sequencing. The local arbuscular mycorrhizae are directly screened from the apple rhizosphere soil through the second-generation high-throughput sequencing technology Fungal dominant species, such as figure 1 , at the genus level ( figure 1 A) and species level ( figure 1 B) All show that the dominant bacteria in the soil sample is Paraglomus sp.

Embodiment 2

[0036] Embodiment 2: Isolation and identification of high-efficiency dominant strain

[0037] 1. Collect soil samples of the rhizosphere of Apple genus in Wantou Village, Shahe Town, Laizhou.

[0038] 2. Mix the collected soil samples with sterilized river sand at a weight ratio of 1:1, put them in pots, and divide them into three parts. The first part is sown with corn, the second part with clover, and the third part with Pingyi sweet tea seedlings ( 3 replicates each), carry out enrichment culture to the dominant bacteria of the genus Glomus, and after 4 months of cultivation in the greenhouse, mix three parts of the soil of sowing corn, clover and Pingyi sweet tea seedlings to prepare the bacterium of the genus Glomus. Isolation of dominant bacteria.

[0039] 3. Take 20g of soil sample from the mixed soil, put it in a 1L beaker, add 500mL of sterile water, stir evenly, and pass through 50 mesh and 400 mesh sampling sieves successively. Transfer all the residue on the 400-...

Embodiment 3

[0050] Embodiment 3: the preparation of SW1 bacterial agent

[0051] The SW1 strain was inoculated on the roots of clover that had grown for one week, and single-spore culture was carried out in the greenhouse for one month, and further multiplied, and the roots of the clover were crushed to obtain a powder containing SW1.

[0052] Mix the sterilized substrate (common seedling substrate, which can be obtained through commercial channels) and the powder containing SW1 at a mass ratio of 3:1, sow clover, and expand the propagation in the greenhouse and potted conditions. Three months later, the infection rate was detected, the shoots were removed, and the roots of the clover were crushed together with the substrate to obtain the SW1 inoculum for subsequent experiments, air-dried in a cool place, and stored at 4°C.

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Abstract

The invention discloses a mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles. The invention isolates and identifies a mycorrhizal fungus belonging to the genus Glomus. Inoculating the mycorrhizal fungus of the genus Glomus genus obtained in the present invention to the roots of apple rootstocks to form rootstocks resistant to repeated cropping obstacles, which can effectively reduce the obstacles to repeated cropping. Compared with the traditional method of cultivating resistant rootstocks, the cultivating efficiency is improved, and the years can be shortened by 7-10 years.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a mycorrhizal fungus of the genus Glomus and its application in the cultivation of rootstocks resistant to apple cropping obstacles. Background technique [0002] The obstacle of repeated cropping is a common phenomenon in the plant world, and the obstacle of repeated cropping of apple is particularly serious. Limited by land resources, in the process of renewal and reconstruction of old orchards in major apple producing areas in my country, 40% to 90% of the orchards must be cultivated with heavy cropping, which will lead to serious pests and diseases, short plants, irregular garden appearance, low yield, and poor varieties, etc. Phenomenon, serious economic loss. [0003] Studies have shown that Fusarium is the main pathogen causing obstacles to apple cropping in my country. Traditional control measures are difficult to apply in production because they are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G17/00A01G2/30A01N63/30A01P21/00C12R1/645
CPCA01N63/30A01G17/005A01G2/30
Inventor 毛志泉王玫尹承苗王功帅吴爱杰段亚楠王海燕相立姜伟涛陈学森沈向
Owner SHANDONG AGRICULTURAL UNIVERSITY
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