The invention discloses a culture medium used for increasing flavonoid compounds and polysaccharides of sculellaria barbata. The culture medium is prepared from the following raw materials: watermelon juice, grape juice, plum fruit juice, muskmelon juice, tea polyphenol, potassium nitrate, ferric phosphate, ammonium nitrate, calcium nitrate, magnesium nitrate, potassium iodate, sodium borate, potassium molybdate, zinc chloride, manganous sulfate monohydrate, copper chloride, ferric nitrate, vitamins, amino acid, indolebutyric acid, gibberellin, 6-benzyl adenine, isopentene aminopurine, naphthylacetic acid, agar, kinetin, cosolvent, a dispersant, a compatilizer, a stabilizer, an antibacterial agent, and water. The culture medium used for increasing the flavonoid compounds and polysaccharides of the sculellaria barbata has the advantages that collocation is reasonable, nutrition is comprehensive, growth of stems on roots can be rapidly promoted, transplantation survival rate is improved, and accumulation of the flavonoid compounds can also be promoted, so that economic benefit is improved; and a preparation method of the culture medium used for increasing the flavonoid compounds and polysaccharides of the sculellaria barbata is easy to operate, and industrialization, standardization and standardized production can be easily realized.