Preparation method of special tissue culture solution for dendrobium candidum

A tissue culture, Dendrobium officinale technology, applied in botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of slow growth cycle, artificial seedling, low survival rate, etc. content, the effect of improving the survival rate

Active Publication Date: 2015-04-29
蒙城县望槐信息科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tender stems of a few varieties such as Dendrobium candidum are twisted into spiral or spring-shaped and dried in the sun. The commercial product is called Dendrobium earrings. Howe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method for preparing a special tissue culture solution for Dendrobium officinale provided by the present invention includes the following steps:

[0019] S1, by weight, weigh out 2.2 parts of potassium iodide, 42 parts of manganese sulfate, 23 parts of zinc sulfate, 72 parts of boric acid, 1 part of sodium molybdate, 0.3 part of copper sulfate, 0.3 part of cobalt chloride, add water to dissolve and stir well to obtain the first Components, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 25:23;

[0020] S2. In moles, weigh 33 parts of ferrous sulfate and 59 parts of disodium ethylenediaminetetraacetic acid, add water to dissolve them, and stir to obtain the second component. The molar volume ratio of ferrous sulfate to the second component (mmol / L) is 33: 1.9;

[0021] S3, weigh 1.6×10 by weight 5 Parts of inositol, 400 parts of niacin, 800 parts of pyridoxine hydrochloride, 170 parts of thiamine hydrochloride, and 1600 parts of glyci...

Embodiment 2

[0025] The method for preparing a special tissue culture solution for Dendrobium officinale provided by the present invention includes the following steps:

[0026] S1, by mass fraction, weigh 2.5 parts of potassium iodide, 40 parts of manganese sulfate, 25 parts of zinc sulfate, 70 parts of boric acid, 1.5 parts of sodium molybdate, 0.1 part of copper sulfate, 0.5 part of cobalt chloride, add water to dissolve and stir evenly to obtain the first Components, wherein the molar volume ratio (mmol / L) of potassium iodide to the first component is 11:12;

[0027] S2, in moles, weigh 30 parts of ferrous sulfate and 62 parts of disodium ethylenediaminetetraacetic acid, add water to dissolve and stir to obtain the second component. The molar volume ratio of ferrous sulfate to the second component (mmol / L) is 10:0.7;

[0028] S3. Weigh 1.4×10 by weight 5 Parts of inositol, 410 parts of niacin, 780 parts of pyridoxine hydrochloride, 190 parts of thiamine hydrochloride, and 1550 parts of glyc...

Embodiment 3

[0032] The method for preparing a special tissue culture solution for Dendrobium officinale provided by the present invention includes the following steps:

[0033] S1, by weight, weigh 2.3 parts of potassium iodide, 41.2 parts of manganese sulfate, 23.7 parts of zinc sulfate, 71.2 parts of boric acid, 1.2 parts of sodium molybdate, 0.22 parts of copper sulfate, 0.36 parts of cobalt chloride, add water to dissolve and stir well to obtain the first Components, wherein the molar volume ratio of potassium iodide to the first component (mmol / L) is 2.4:2.35;

[0034] S2. In moles, weigh 32 parts of ferrous sulfate and 60 parts of disodium ethylenediaminetetraacetic acid, add water to dissolve them, and stir to obtain the second component. The molar volume ratio of ferrous sulfate to the second component (mmol / L) is 32: 1.9;

[0035] S3, weigh 1.56×10 by weight 5 Parts of inositol, 403 parts of niacin, 795 parts of pyridoxine hydrochloride, 175 parts of thiamine hydrochloride and 1590 pa...

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PUM

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Abstract

The invention discloses a preparation method of a special tissue culture solution for dendrobium candidum. The preparation method comprises the following steps: dissolving potassium iodide, manganese sulfate, zinc sulfate, boric acid, sodium molybdate, copper sulfate and cobalt chloride to obtain a first component; dissolving ferrous sulfate and disodium edetate dehydrate to obtain a second component; dissolving inositol, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine to obtain a third component; weighing peeled potatoes, adding the potatoes in a food processer, stirring to obtain mashed potato, adding coconut milk in the mashed potato, and uniformly mixing to obtain a material A; adding ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate and monopotassium phosphate in water to dissolve to obtain a material B; heating the material A, and adding the material B when the material A is heated, uniformly stirring, sequentially adding the first component, the second component and the third component, continuously adding brown sugar and agar, uniformly stirring, and regulating a pH value to obtain a mixed material; packaging the mixed material to sterilize at a high temperature and under high pressure, cooling the sterilized material to a room temperature to obtain the special tissue culture solution for the dendrobium candidum.

Description

Technical field [0001] The invention relates to the technical field of plant culture, in particular to a method for preparing a special tissue culture solution for Dendrobium candidum Background technique [0002] Dendrobium is a perennial epiphytic herb of the genus Dendrobium of Orchidaceae, and it is a precious traditional Chinese medicinal material commonly used in my country. As early as the "Shen Nong's Materia Medica" and "Compendium of Materia Medica", it was listed as top-grade medicinal materials. Dendrobium is used as medicine with stems and contains many alkaloids such as dendrobine, dendrobium, dendrobine, dendrobium, etc; Cardiovascular and ophthalmological diseases have obvious therapeutic effects. Modern medicine has proved that Dendrobium can promote blood circulation and enhance heart function; promote gastrointestinal motility and enhance digestion; it can significantly increase the level of SOP (superoxide dismutase) in the human body and reduce LPO (lipid p...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李军
Owner 蒙城县望槐信息科技有限责任公司
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