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A kind of bacterium and mof base carrier hybrid material and its preparation method and application

A hybrid material and MOF technology, which is applied in the field of hybrid materials of bacteria and MOF-based carriers and their preparation, can solve the problems of single treatment mode and poor accuracy of traditional nano-therapeutics, so as to enhance the curative effect of tumor treatment and improve the efficiency of tumor inhibition. , the effect of reducing off-target side effects

Active Publication Date: 2022-06-07
WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional nanotherapeutics have the defects of poor precision and single treatment mode. Therefore, how to develop a kind of nanomaterial with high precision and multiple anti-tumor treatment modes has become an urgent technical problem to be solved.

Method used

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  • A kind of bacterium and mof base carrier hybrid material and its preparation method and application
  • A kind of bacterium and mof base carrier hybrid material and its preparation method and application
  • A kind of bacterium and mof base carrier hybrid material and its preparation method and application

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preparation example Construction

[0045] According to another exemplary embodiment of the present invention, there is provided a method for preparing a hybrid material of a bacterium with a MOF-based carrier, such as Figure 6 As shown, the method comprises:

[0046] S1, moF nanoparticles loaded with therapeutic agents, to obtain a load of chemotherapy drugs MOF carrier;

[0047] As an alternative embodiment, the MOF nanoparticles are synthesized using controlled titration, comprising:

[0048] MIL-101-NH was synthesized by controlled titration 2 (Fe) MOF, as follows: the 10~ 50mM metal precursor solution and 10~50mM ligand precursor solution were added to the reactor containing 8~16mL DMF / water mixed solvent by the syringe pump at 10~30mL / h through a separate pipeline, and the reaction was carried out under stirring at 50 °C. After the reaction is completed, the reaction solution is removed, centrifuged (10000~12000rpm, 10~20min), washed to obtain MIL-101-NH with morphology and regular size 2 (Fe) MOF, i.e. the MO...

Embodiment 1

[0089] Example 1 Khiva bacteria bind iron-based MOF hybrid active material and preparation method thereof

[0090] (1) The metal precursor solution (40mM of aqueous iron chloride solution) and the ligand precursor solution (40mM of 2-amino-terephthalic acid DMF / water (V / V = 1 / 1) solution) are configured, and then the above-described precursor solution is added by the syringe pump through a separate pipeline at 20mL / h to the reactor containing 10mL DMF / water (1 / 3) mixed solvent, and the reaction is 24h under stirring at 50 ° C. Subsequently, centrifugation (11,000 rpm, 15min) and washing yielded MIL-101-NH with morphology and dimensions 2 (Fe) MOF。

[0091] (2) The above (1) yielded 10mg MIL-101-NH 2 (Fe) MOF is evenly dispersed in 10 mL aqueous solution and sonicated for 10 min to make it fully dispersed. Subsequently, an aqueous solution of 1 mL DOX (0.5 mg / mL) was added to the above-mentioned nano MOF dispersion, stirred at room temperature to avoid light for 24 h, an...

Embodiment 2

[0096] Example 2 Khiva bacteria bind iron-based MOF hybrid active material and preparation method thereof

[0097] (1) The metal precursor solution (10mM aqueous iron chloride solution) and the ligand precursor solution (10mM of 2-amino- terephthalic acid DMF / water (V / V = 1 / 1) solution) are configured), and then the above-described precursor solution is added by the syringe pump through a separate pipeline at 10mL / h to a reactor containing 8mLDMF / water (1 / 3) mixed solvent, and the reaction is 24h at 50 °C stirring. Subsequently, centrifugation (11,000 rpm, 15min) and washing yielded MIL-101-NH with morphology and dimensions 2(Fe) MOF。

[0098] (2) The above (1) resulted in 5 mg MIL-101-NH 2 (Fe) MOF is evenly dispersed in 10 mL aqueous solution and sonicated for 10 min to make it fully dispersed. Subsequently, an aqueous solution of 1 mL DOX (0.1 mg / mL) was added to the above-mentioned nano MOF dispersion, stirred at room temperature to avoid light for 24 h to obtain MIL...

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Abstract

The invention discloses a bacteria-MOF-based carrier hybrid material and its preparation method and application. The method includes: loading MOF nanoparticles with a therapeutic agent to obtain a MOF carrier loaded with chemotherapy drugs; wrapping hyaluronic acid on the loaded Chemotherapeutic drug MOF carrier, obtain surface negatively charged MOF carrier; Polyallylamine hydrochloride is modified on the surface of Shewanella, obtain surface positively charged Shewanella solution; Described surface negatively charged MOF carrier and described The surface-positive Shewanella solution was combined by electrostatic interaction to obtain the hybrid material of bacteria and MOF-based carrier. Bacteria and MOF-based carrier hybrid materials are used in anti-tumor therapy with high precision and multi-modal combination therapy. It not only combines the advantages of each single therapy, but also can make up for their respective shortcomings, resulting in an efficient and low-toxic anti-tumor effect. .

Description

Technical field [0001] The present invention relates to the field of biomedical technology, particularly to a bacterium and MOF-based carrier hybrid material and preparation method and application thereof. Background [0002] As one of the deadliest diseases in the world, cancer has seriously threatened human life and health. At present, chemotherapy, radiation therapy, surgical therapy and high-intensity focused ultrasound therapy have been widely used in the clinic and have achieved great success in inhibiting tumor proliferation and prolonging patient survival. However, due to the complexity, diversity, and heterogeneity of tumors, current clinical treatments have achieved only limited success. For example, in order to meet the needs of malignant proliferation of tumor cells, tumor cells often exhibit abnormal energy metabolism characteristics, mainly manifested by greatly increasing glucose uptake and enhancing glycolysis, which eventually leads to a large accumulation of met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/46A61K47/22A61K47/36A61K47/32A61K35/74A61K31/704A61K31/337A61K41/00A61P35/00
CPCA61K47/46A61K47/22A61K47/36A61K47/32A61K35/74A61K31/704A61K31/337A61K41/0071A61P35/00A61K2300/00Y02A50/30
Inventor 陈巍海梁军龙王佳玮雒国凤张先正
Owner WUHAN UNIV
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