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Application of enol substances in pine needles in aspects of regulating and controlling panax notoginseng rhizosphere microflora structure

A technology of microbial community and middle enol, which can be applied in the fields of application, plant protection, fertilizer mixture, etc., and can solve problems such as continuous cropping obstacles of Panax notoginseng

Active Publication Date: 2021-09-07
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem of Panax notoginseng continuous cropping obstacles, improve the planting and growth environment of Panax notoginseng, and improve the quality of Panax notoginseng, the invention provides a kind of enol substances in pine needles that can regulate the structure of the microbial community of Panax notoginseng rhizosphere and / or repair the continuous cropping of Panax notoginseng Carrier and / or application in improving Panax notoginseng planting

Method used

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  • Application of enol substances in pine needles in aspects of regulating and controlling panax notoginseng rhizosphere microflora structure
  • Application of enol substances in pine needles in aspects of regulating and controlling panax notoginseng rhizosphere microflora structure
  • Application of enol substances in pine needles in aspects of regulating and controlling panax notoginseng rhizosphere microflora structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: α-蒎 蒎 调 三 三 结构 结构 结构 试 试 试

[0065] This test uses a closed space fumigation method: placed the three or seven pot plants after transplanted survival in a transparent plastic sealing incubator, add 0 and 5 μl / L of α-α-α-蒎 (α-蒎 蒎 volume / incubator) per box, The fumigation time was 3d, and then collected the rooted soil of potted three seven. After 60 mesh sieve, the change in soil microorganism was determined by a plate method, and the bacterial culture was used as a beef paste protein culture, and the fungi culture was used in Bangladesh red culture. The soil sample 5g, which is sieved, is accurately referred to by electronic balance, 5G in the 50 mL centrifuge tube containing 45 ml of sterile water, placed on the shaker (120R / min, normal temperature), oscillated 20 minutes, soil soil sufficiently dispersed into a soil Suspension, dilution is 10 -1 . Pipeline from 10 -1 Dilution solution was taken in a test tube containing 9 ml of sterile water in the diluti...

Embodiment 2

[0067] Example 2: D-pine alcohol and 2,3-butanediol regulate three seven-channel microbial community structural test

[0068] This test uses a closed space spraying method: placed three or seven pot plants after transplanted survival in transparent plastic sealing incubator, 10 plant three seven per pot, each incubator is loaded into 6 pots, and the three or seven-leaves spray 100ml The sterile water, 1 mg / L of D-pqf alcohol and 1 mg / L of 2,3-butanediol three different treatments, spraying sterile water as a control treatment, spraying once a day, lasting 5D, after collecting potted three The rhizosphere soil, after 60 mesh sieve, the change in soil microorganisms was determined by the plate method, and the bacterial culture was used as a beef paste protein culture medium, and the fungi culture was used in Bangladesh red culture. The soil sample 5g, which is sieved, is accurately referred to by electronic balance, 5G in the 50 mL centrifuge tube containing 45 ml of sterile wat...

Embodiment 3

[0070] Example 3: 2,3-butanediol regulatory link soil microbial community structure test

[0071] This test uses a long-term water for the pot: Sanqi, serves as a soil-seeking mountain three-seven farmland, and will take it back and carry out the dry treatment, then 40 mesh sieves, remove plant residues in the soil, weigh 4 The serial bouting of the kg is added to the storage box (28 × 20 × 17 cm); 1 ml of analyzing pure 2,3-butanediol is added to 1L sterile water, and is equipped into a 1000 ppm mother liquor, and then gradually diluted into 1, 10, 50 100 ppm solution, with sterile water as a control treatment; 500ml of the above solution is added to different storage boxes, then cover the box cover, and seal it with a sealing film, place the boxes in a cool place, each treatment Set 3 repetitions. After 1 month, the dilution coating plate method was used to statistically analyze the number of soil microorganisms. Take 5g of soil-free 50 ml of 50 ml of centrifuge tube (120R / min...

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Abstract

The invention relates to application of enol substances in pine needles in the aspects of regulating and controlling a panax notoginseng rhizosphere microflora structure and / or repairing a panax notoginseng continuous cropping carrier and / or improving panax notoginseng planting. What discovers is that the quantity of fungi in panax notoginseng rhizosphere soil or panax notoginseng continuous cropping soil can be reduced and the quantity of bacteria can be obviously increased by treating potted panax notoginseng or soil by using rich substance components alpha-pinene, 2, 3-butanediol and D-pinitol in a fumigation, spraying or irrigation manner, therefore, the ratio of fungi to bacteria in the soil is remarkably reduced, and the soil is converted from a fungal type into a bacterial type, so that planting and growth of panax notoginseng are facilitated.

Description

Technical field [0001] BACKGROUND OF THE INVENTION 1. Specifically, the present invention relates to applications of pine needles in the regulation of three seven-channel microbial community structures. Background technique [0002] Three seven (Burk.) F.h.chen) is also known as the seven, gold does not change, and the five-Cankee will participate in the perennial hugage grass plant. The three seven have the effect of taking bleeding and swelling, and has been favored since ancient times, and there have been more than 400 years of domesticated cultivation history. At present, the Sanqi Production Area is concentrated in Wenshan, Yoshan, Maguan, Guangnan, Qiu Bei, Missi, Malang, Guangnan, Qiu Bei, Siwei, Malang, Guangnan, Qiu Bei, Jianwei, Shipou, screen, Shilin, and Guangxi Zhuang Autonomous Region Jingjing Western counties and cities, the production of three hundred tons per year, the output value is close to 100 billion yuan. [0003] However, the three seven of severely facili...

Claims

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Application Information

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IPC IPC(8): C09K17/14A01B79/02A01G13/00A01G22/25
CPCC09K17/14A01G13/00A01B79/02A01G22/25
Inventor 朱书生张俊星叶辰李天尧黄惠川刘屹湘杨敏梅馨月邓维萍何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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