Ex-vivo blood sample analysis method and immunity evaluation device

A technology of blood samples and analysis methods, which is applied in the field of analysis of immunity evaluation devices and isolated blood samples, can solve the problems of no reference value, etc., and achieve the effect of comprehensive, accurate and quantitative evaluation at the overall level

Pending Publication Date: 2021-09-24
河北森朗泰禾生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

For sub-healthy people, cancer patients, and patients with cured tumors, these examinations have no reference value

Method used

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  • Ex-vivo blood sample analysis method and immunity evaluation device
  • Ex-vivo blood sample analysis method and immunity evaluation device
  • Ex-vivo blood sample analysis method and immunity evaluation device

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Embodiment Construction

[0092] In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0093] The present invention is divided into following several parts:

[0094] (1) Lymphocyte isolation, lymphocyte stimulation and detection

[0095] The reagents and sources and storage conditions to be used in this step are as follows:

[0096]

[0097] 1. Use conventional methods to separate lymphocytes by density centrifugation, take 10ml of venous blood from the subject, put it in a purple-capped EDTA anticoagulant tube, and use human lymphocyte separation medium (Dongfang Huahui, product number 25610) to separate lymphocytes. The specific steps of separation are as follows:

[0098] (1) Take three 50ml centrifuge tubes and label them with ①separation solution, ②blood sample, and ③PBMC.

[0099] (2) Add 15ml of human mononuclear cell separation medi...

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Abstract

The invention relates to an ex-vivo blood sample analysis method. The method comprises the steps of S1 separating lymphocytes from ex-vivo blood provided by a subject, culturing the lymphocytes in vitro for stimulation and cell staining, detecting specific surface protein on the surface of a single lymphocyte in the lymphocytes, and detecting and calculating the number and proportion of various cells according to positive or negative results displayed by different specific surface proteins; S2 performing standardized scoring on the number and the proportion of each type of cells to obtain a corresponding standard score; S3 classifying the functions represented by the cell types corresponding to the scores into six types, namely T cell reserve, an inherent immune system, an NK cell killing function, T cell balance, T cell activation and T cell functions; summarizing the scores of the index items contained in each type to obtain a total score of the type; and S4 summing the six types of total scores of a testee to obtain an analysis result corresponding to the blood sample of the certain testee.

Description

technical field [0001] The invention relates to the technical field of isolated blood sample analysis, in particular to an analysis method and an immunity evaluation device for isolated blood samples. Background technique [0002] The immune system develops and matures during the growth process, and the overall immune cell number, proportion, and functional level present a steady state in a healthy human body. But as we age, some cells enter the senescence stage, and the immune system becomes out of balance. Imbalances in the immune system often do not change significantly at the initial stage, and are not noticed until the physical condition deteriorates and diseases (infections, tumors) occur, but further intervention often takes more time and money. On the other hand, for cancer patients who have achieved remission through existing treatments, there is a need for a means of early warning of the possibility of recurrence in addition to imaging and tumor markers. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/30
CPCG01N33/48G01N1/30
Inventor 何晋元李建强巴敏李超龙杨星齐立婓吴雪李泽坤程高满
Owner 河北森朗泰禾生物科技有限公司
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