Application of soybean gene promoters peif1 and peif1-i in soybean, Arabidopsis and tobacco

A promoter and Arabidopsis technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of imperfect screening conditions for constitutive promoters, gene silencing, and no expression related to soybean nodule development
CN113462689BActive Publication Date: 2022-06-21HENAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN UNIVERSITY
Publication Date
2022-06-21

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Abstract

The invention belongs to the technical field of plant genetic engineering, in particular to a soybean gene promoter pEIF1 and pEIF1‑I Application in soybean, Arabidopsis and tobacco. The present invention clones the gene from soybean RPS28 and genes EIF1 ; Get the promoter by CTAB method pEIF1 and promoter pEIF1‑I , and then construct a soybean stable transformation vector, and realize pEIF1 and pEIF1‑I The expression of the driven GUS protein at each stage of soybean genetic transformation, found that pEIF1 , pEIF1‑I The driven GUS is expressed in cotyledons, radicles, germs, true leaves, compound leaves, buds, petioles, internodes, roots and nodules, indicating that pEIF1 and pEIF1- I It is a ubiquitous expression promoter, and the activity of GUS protein expression product in soybean is determined. In addition, it also implements pEIF1 and pEIF1‑I Driving transformation of GUS proteins in Arabidopsis and tobacco transformation.
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Description

technical field

[0001] The invention belongs to the technical field of plant genetic engineering, in particular to a soybean gene promoter pEIF1 and pEIF1-I Application in soybean, Arabidopsis and tobacco. Background technique

[0002] Promoters are an important part of regulating plant gene expression. According to the source, promoters can be divided into exogenous promoters and endogenous promoters. Exogenous promoters can heterologously regulate gene expression, but transgenic safety problems often arise when preparing transgenic materials, especially commonly used promoters derived from viruses, such as the tobacco mosaic virus 35S promoter. In addition, transgene silencing may occur when the same promoter is used in the same transgenic event. Therefore, endogenous promoters are more favored promoters for the preparation of transgenic materials. According to the expression characteristics, promoters are divided into constitutive promoters, tissue-specific promoter...

Claims

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