Heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa

A technology of chlorella pyrenoidosa and its cultivation method, which is applied in the field of heterotrophic-dilution-light-induced cultivation of chlorella pyrenoidosa, which can solve the problem of low protein content of heterotrophic cultured algae flour, lack of culture conditions and culture techniques, and production problems. Long cycle and other issues, to achieve the effect of improving comprehensive utilization value, facilitating large-scale promotion and application, and easy operation

Inactive Publication Date: 2021-10-12
新疆金正生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Cultivation conditions and cultivation techniques are relatively lacking. In order to solve the problems of long autotrophic culture production cycle, low biomass and low protein content of heterotrophic culture alga

Method used

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  • Heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa
  • Heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa
  • Heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa

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Embodiment 1

[0023] A kind of heterotrophic-dilution-light-induced culture method of Chlorella desert pyrenoidosa of the present invention, the technical scheme adopted is, comprises steps as follows:

[0024] Step 1. Activation of algae species: Take out the streaked Chlorella pyrenoidosa from the algae resource bank, pick a single algae strain in a sterile workbench, streak it in a solid medium, and place it in a light incubator for cultivation. The culture medium is Basal solid medium containing 10g / L glucose, the culture temperature is 28°C, and the light is 5000lux for 15 days to obtain activated algal species;

[0025] Step 2, preparation of primary seed liquid: pick 3-ring algae species from the activated algae species, inoculate into a 100mL Erlenmeyer flask with 50mL medium, the medium is Basal solid medium containing 10g / L glucose, at temperature 28 ℃, light 5000lux, 50r / min shaking incubator culture to logarithmic growth phase 10 8 individual / mL to obtain the primary seed solut...

Embodiment 2

[0031] A kind of heterotrophic-dilution-light-induced culture method of Chlorella desert pyrenoidosa of the present invention, the technical scheme adopted is, comprises steps as follows:

[0032] Step 1. Activation of algae species: Take out the streaked Chlorella pyrenoidosa from the algae resource bank, pick a single algae strain in a sterile workbench, streak it in a solid medium, and place it in a light incubator for cultivation. The culture medium is Basal solid medium containing 10g / L glucose, the culture temperature is 28°C, and the light is 5000lux for 15 days to obtain activated algal species;

[0033] Step 2, preparation of primary seed liquid: pick 5-ring algae species from the activated algae species, inoculate into a 100mL Erlenmeyer flask with 50mL medium, the medium is Basal solid medium containing 10g / L glucose, at temperature 28 ℃, light 5000lux, 50r / min shaking incubator culture to logarithmic growth phase 10 8 individual / mL to obtain the primary seed solut...

Embodiment 3

[0039] A kind of heterotrophic-dilution-light-induced culture method of Chlorella desert pyrenoidosa of the present invention, the technical scheme adopted is, comprises steps as follows:

[0040] Step 1. Activation of algae species: Take out the streaked Chlorella pyrenoidosa from the algae resource bank, pick a single algae strain in a sterile workbench, streak it in a solid medium, and place it in a light incubator for cultivation. The culture medium is Basal solid medium containing 10g / L glucose, the culture temperature is 28°C, and the light is 5000lux for 15 days to obtain activated algal species;

[0041] Step 2, preparation of primary seed liquid: pick 5-ring algae species from the activated algae species, inoculate into a 100mL Erlenmeyer flask with 50mL medium, the medium is Basal solid medium containing 10g / L glucose, at temperature 28 ℃, light 5000lux, 50r / min shaking incubator culture to logarithmic growth phase 10 8 individual / mL to obtain the primary seed solut...

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Abstract

The invention relates to a heterotrophism-dilution-light induction culture method for desert chlorella pyrenoidosa. According to the method, a heterotrophic mode and an autotrophic mode are combined, so that the biomass and the protein content of the desert chlorella pyrenoidosa are greatly improved, the purpose of efficiently producing a large amount of desert chlorella pyrenoidosa with high protein content is achieved, the chlorella pyrenoidosa cell biomass with relatively high concentration can be obtained in a short time, the accumulation of substances such as protein and chlorophyll in the chlorella pyrenoidosa can be induced, the quality of the chlorella pyrenoidosa is improved, and the comprehensive utilization value of the chlorella pyrenoidosa is increased. Besides, the method has the characteristics of simplicity and convenience in operation, high practicability, easiness in realization of continuous production and the like, and is very beneficial to large-scale popularization and application, so that a large amount of desert chlorella pyrenoidosa powder with high protein content can be quickly and efficiently obtained, the technical support is provided for production of the high-quality desert chlorella pyrenoidosa powder, and the requirements of industrial production are met.

Description

technical field [0001] The invention relates to the technical field of microalgae culture, in particular to a heterotrophic-dilution-light-induced culture method for Chlorella pyrenoidosa. Background technique [0002] Chlorella sp. is a general unicellular green alga belonging to Chlorophyta, Chlorophyceae, Oocystaceae, Chlorella genus. The common types of chlorella in my country include Chlorella pyrenoidosa, Chlorella ellipsoides, and Chlorella vulgaris. Chlorella is rich in protein, unsaturated fatty acids (linoleic acid, linolenic acid, DHA, EPA, etc.), carotenoids, astaxanthin and multivitamins, and is widely used in functional food, feed, cosmetics and medicine. [0003] The existing production methods of Chlorella pyrenoidosa algae powder mainly include open autotrophic culture, closed heterotrophic culture and mixed culture. Open autotrophic culture is easy to pollute, light, temperature and other culture conditions are easily affected by weather, the concentratio...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N13/00C12R1/89
CPCC12N1/12C12N13/00
Inventor 梁钧
Owner 新疆金正生物科技有限公司
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