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Helicobacter pylori staining kit and application

A technology of Helicobacter pylori and dyeing reagents, applied in the field of biomedicine, can solve problems such as long time, achieve the effects of improving efficiency, reducing the probability of false positives and false negatives, good application prospects and high social and economic value

Pending Publication Date: 2021-10-15
河南赛诺特生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under the microscope, Helicobacter pylori infection can be observed through HE slices, but the diagnosis doctor requires more experience and is not as accurate as the results of immunohistochemistry or special staining experiments
At the same time, do HE first and then do immunohistochemistry or special staining. The report will take at least 2-3 days, which will cause a certain economic and mental burden on the patient.

Method used

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  • Helicobacter pylori staining kit and application
  • Helicobacter pylori staining kit and application
  • Helicobacter pylori staining kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Helicobacter pylori staining kit

[0041] This embodiment provides a kind of Helicobacter pylori staining kit, is made of following components:

[0042] 1) Peroxidase blocking agent, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: SD3002;

[0043] 2) Helicobacter pylori monoclonal antibody, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: CHR-0171;

[0044] 3) Mouse anti- or rabbit anti-Linker, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: SD3010;

[0045] 4) Enzyme-labeled goat anti-mouse / rabbit polymer, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: SD3003;

[0046] 5) DAB chromogenic substrate, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: SD3004;

[0047] 6) DAB chromogenic buffer, purchased from Henan Sinotech Biotechnology Co., Ltd., item number: SD3005;

[0048] 7) Hematoxylin counterstaining solution, purchased from Henan Sinotech Biotechnolog...

Embodiment 2

[0053] Application of embodiment 2 in pathological tissue staining

[0054] This embodiment provides the application of the kit of Embodiment 1 in pathological tissue staining, the sample to be tested is a gastric biopsy sample or a gastric tissue wax block section, specifically comprising the following steps:

[0055]1) Slicing: Put the slices into a slicer and bake at 65°C for 2 hours.

[0056] 2) Airborne staining: prepare the sample to be tested, load the Helicobacter pylori staining kit on the automatic immunohistochemical staining machine for staining; set the CNT330 staining program, print and label the test operation, and the specific airborne staining program as follows:

[0057] a) Onboard dewaxing and antigen retrieval, and cleaning with onboard cleaning solution;

[0058] b) Block with peroxidase for 5 minutes; wash with on-board cleaning solution for 3 times, 2 minutes each time;

[0059] c) Incubate with the Helicobacter pylori monoclonal antibody for 30 minut...

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Abstract

The invention relates to a helicobacter pylori staining kit and application. The kit comprises a peroxidase blocking agent, a helicobacter pylori monoclonal antibody, a mouse-resistant or rabbit-resistant Linker, an enzyme-labeled goat-anti-mouse / rabbit polymer, a DAB chromogenic substrate, a DAB chromogenic buffer solution, a hematoxylin restaining solution, a blue-returning solution, a weak acid salt buffer solution and an eosin staining solution. The kit can meet the requirements of immunohistochemical staining and conventional HE staining at the same time, and the information amount of a single piece is remarkably increased; in addition, a weak acid salt buffer solution is added in the kit, so that the kit is suitable for a full-automatic immunohistochemical machine. When the kit is used for pathological tissue dyeing, high-flux full-automatic airborne dyeing can be realized, and the manpower and time cost is greatly reduced. Tests prove that the kit improves the sensitivity and specificity of helicobacter pylori staining, reduces the occurrence probability of false positive and false negative, further improves the repeatability of results and the accuracy of interpretation, and has good application prospects and high social and economic values.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a Helicobacter pylori staining kit and its application. Background technique [0002] Gastric cancer is one of the most common malignant tumors of the digestive system, ranking first among malignant tumors of the digestive system, with low survival rate and poor prognosis. And Helicobacter pylori has been proved to be an important carcinogen causing gastric cancer; Helicobacter pylori (HP) infection first causes chronic gastritis and leads to gastric ulcer and gastric atrophy, and severe cases can develop into gastric cancer. Therefore, it is particularly important to accurately detect HP clinically. [0003] Helicobacter pylori is a unipolar, multi-flagellated, blunt-ended, helically curved bacterium. 2.5-4.0 μm long and 0.5-1.0 μm wide. Gram stain negative. Motivated. On the surface of gastric mucosal epithelial cells, it often presents a typical spiral or a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31G01N33/531G01N33/577
CPCG01N1/31G01N33/531G01N33/577
Inventor 米贯勋崔红米赵琳琳邢亚楠牛银银李三华刘文弟齐华
Owner 河南赛诺特生物技术有限公司
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