Preparation of double-index immunochromatography kit for detecting sST2/Gal3

An immunochromatography test strip and immunochromatography technology, applied in the field of immunology, can solve the problems of missing injury or inflammation sites, insufficient sensitivity of cardiac biopsy methods, etc., achieve high specificity, meet clinical testing requirements, and simple operation

Pending Publication Date: 2021-10-22
武汉市朗典精医生物科技有限公司
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Problems solved by technology

[0003] At present, there is no specific biomarker for screening or diagnosis of myocarditis. The gold standard for diagnosis of myocarditis is through cardiac biopsy to verify the existence of myocardial tissue damage and inflammation. However, different myocardial parts suffer from different degrees of damage and inflammation, and biopsy may miss damage. Or the site of inflammation, the cardiac biopsy method is not sensitive enough, the positive rate is only 30%, it is necessary to find a new method for the diagnosis of myocarditis

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  • Preparation of double-index immunochromatography kit for detecting sST2/Gal3
  • Preparation of double-index immunochromatography kit for detecting sST2/Gal3
  • Preparation of double-index immunochromatography kit for detecting sST2/Gal3

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Embodiment Construction

[0030] In conjunction with specific experiments, the principles and results of the present invention are further described, and the multi-step experimental process of the present invention is listed below.

[0031] 1. Preparation of sST2 / Gal3 immunochromatography kit

[0032] The sST2 / Gal3 immunochromatography kit includes immunochromatography test strips, chromatography fluid and sST2 / Gal3 calibrator, and the preparation process is as follows.

[0033] (1) Preparation of sST2 antibody-fluorescent microspheres

[0034] In the embodiment of the present invention, europium chelate (Europium Chelate) fluorescence-labeled carboxyl-modified microparticles are used, which are purchased from Bangs Laboratories Inc. The diameter of the microspheres is 100nm-300nm.

[0035] In the embodiment of the present invention, the sST2 monoclonal antibody was purchased from Medix Biochemica and coupled to europium chelate fluorescently labeled carboxyl-modified microspheres with a diameter of 2...

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Abstract

The invention particularly relates to establishment of an immunochromatography kit for simultaneously detecting soluble ST2 (sST2) and beta-galectin-3 (Gal3, Gal3), the main component of the immunochromatography kit is an immunochromatography test strip, and the immunochromatography test strip is formed by sequentially adhering Fusion5, a nitrocellulose membrane and absorbent paper on a PVC bottom plate. The Fusion 5 adsorbs sST2 antibody-microspheres and Gal3 antibody-microspheres, the nitrocellulose membrane fixes the sST2 antibody to form a detection line 1 (T1 line), fixes the Gal3 antibody to form a detection line 2 (T2 line), and fixes the anti-IgG antibody to form a quality control line (C line). The invention also discloses a process for coupling the sST2 antibody and the Gal3 antibody with the microspheres. The sST2/Gal3 immunochromatography kit is established to detect the concentration of sST2 and Gal3 in blood or other body fluids at the same time, and is used for diagnosing myocarditis and monitoring the progress of myocarditis.

Description

technical field [0001] The invention belongs to the field of immunology, and specifically relates to the process of preparing sST2 antibody-fluorescent microspheres and Gal3 antibody-fluorescent microspheres and establishing a sST2 / Gal3 immunochromatographic kit. Background technique [0002] Myocarditis is an inflammatory reaction that damages the myocardium, affects the contractility of the heart muscle and the electrical conduction system of the heart, reduces the heart's pumping ability or causes arrhythmia, and causes heart failure. It is also one of the main causes of sudden and accidental death in children or young people . Viral infection is the main cause of myocarditis, followed by drug-induced myocardial inflammation. Symptoms in patients with myocarditis range from influenza-like illness to dilated cardiomyopathy and heart failure. [0003] At present, there is no specific biomarker for screening or diagnosis of myocarditis. The gold standard for diagnosis of m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543G01N33/558G01N33/577
CPCG01N33/533G01N33/54313G01N33/558G01N33/577
Inventor 杨琦
Owner 武汉市朗典精医生物科技有限公司
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