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Ginger reference genes and application thereof

An internal reference gene and gene technology, applied in the field of molecular biology, can solve the problems of restricting the research progress of molecular breeding of ginger, and the internal reference gene of ginger has not been found, and achieve the effects of shortening detection time, improving reliability, and optimizing PCR amplification procedures.

Active Publication Date: 2021-10-26
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no reports on the related research of internal reference genes in ginger, which seriously restricts the research progress of ginger molecular breeding

Method used

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  • Ginger reference genes and application thereof
  • Ginger reference genes and application thereof
  • Ginger reference genes and application thereof

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Experimental program
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Effect test

Embodiment

[0015] The present embodiment has selected the following three varieties: Shandong large ginger, crested ginger and Yunnan Luoping yellow ginger; four parts of each variety are sampled: root, rhizome, stem, leaf; and each sampling part is divided into The following five treatment groups: drought, flooding, high temperature, low temperature, and salt stress; the RNA of ginger in each treatment group was extracted by Trizol method. The results showed that the stem RNA content was the lowest in each treatment group and among the three varieties, and the comprehensive performance of Crested Ginger was better.

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Abstract

The invention relates to the technical field of molecular biology, and discloses a group of ginger reference genes, and the ginger reference genes are an RBP gene, an ATPase gene and a 40SS3 gene. Application is further disclosed. The reference genes disclosed by the invention are high in stability, and provide more possibilities for the research of a ginger functional gene.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a group of ginger internal reference genes and applications thereof. Background technique [0002] Ginger (Zingiber officinale Roscoe) is a perennial herbaceous plant belonging to the genus Zingiberaceae (Zingiberaceae) of the class Monocotyledoneae, order Zingiberales. It has a long history of cultivation in my country and is widely distributed. Except for the alpine regions such as northeast and northwest, it is cultivated in other provinces and cities. The cultivation is relatively simple and convenient. Ginger not only has medicinal value such as anti-tumor, anti-oxidation, anti-inflammatory and antibacterial, promotes digestion, improves blood circulation, but also has edible value as a condiment or edible vegetable. With the development of market economy and foreign trade, ginger not only has a large sales volume in the country, but also is often processed into d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/29C12N15/55
CPCC12Q1/6895C12Q2600/158C12Q2600/166
Inventor 尹军良朱永兴胡海骏李港蒋昕晨刘弈清
Owner YANGTZE UNIVERSITY
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