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Result determination in an immunoassay by measuring kinetic slopes

A technology to determine the end point and rate, which is applied in the field of determination and measurement, and can solve problems such as errors

Pending Publication Date: 2021-10-29
QUIDEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An error occurs, for example, when samples exhibit widely varying reaction times in a population of positive samples

Method used

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  • Result determination in an immunoassay by measuring kinetic slopes
  • Result determination in an immunoassay by measuring kinetic slopes
  • Result determination in an immunoassay by measuring kinetic slopes

Examples

Experimental program
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Embodiment Construction

[0024] The methods described herein are applicable to any type of endpoint assay, in particular immunoassays, such as enzyme-linked immunosorbent assays (ELISA) or lateral flow assays, and rapid diagnostic strip-based assays, and are independent of antibody / antigen pairs. Other types of assays to which this method can be applied generally include chemical assays, microbiological assays, and biological assays.

[0025] The methods described herein are applicable to a variety of bonding measurements and can be based on measurable signals from sources such as radioactive decay, fluorescence or chemiluminescence. Measurements from other techniques such as colorimetry, photometry, spectrophotometry and chromatography may also be used.

[0026] The methods described herein can be applied in situ or remotely to determine qualitative or quantitative results of assays on test samples.

[0027] In the methods described herein, measurements are taken at multiple time points during the c...

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Abstract

A system including a sample receptacle configured to receive a test sample and a test strip coupled to the sample receptacle is provided. The test strip configured to generate a signal based on a concentration of a target analyte in the test sample. The system also includes a detector to generate a transduced signal based on the signal and a computer to receive a transduced signal. The computer further determines the concentration of the target analyte in the test sample. For this, the computer retrieves the transduced signal from the detector at multiple time points to determine a signal rate based on a signal value for the time points, and to determine the concentration of the target analyte based on the signal rate and a model. A method and a non-transitory, computer-readable medium storing instructions to use the above system are also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims priority under 35 USC §119(e) to US Provisional Patent Application No. 62 / 818,403, filed March 14, 2019, which is hereby incorporated by reference in its entirety for all purposes. technical field [0003] The techniques described herein relate generally to assay measurements, and more specifically to the quantitative determination of endpoints in assay measurements. Background technique [0004] Immunoassays are widely used to detect the presence and / or concentration of analytes, such as antibodies, in a test sample. Most such assays rely on specific bonding between antibody and antigen. In an endpoint assay, only one data point is collected - say 5 minutes after the start of the assay, at which point the lab staff collects the data or develops a bar. Results are characterized as positive or negative readings. In this type of assay, it is assumed that the resulting concentration of the molec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/557G01N33/558
CPCG01N33/557G01N33/54388G01N21/274G01N21/8483G01N2021/6439G01N2201/067
Inventor P·Y-G·任S·霍尔舍尔C·阿尔贝托S·派恩多J·麦克卢尔D·杰斯瓦尔
Owner QUIDEL