Polyphenol modified liposome nano system as well as preparation method and application thereof
A liposome and nanotechnology, applied in the fields of medical health and biomedical materials, can solve the problems of affecting the long-term effect of growth factors, weakening its own stability, difficult to ensure safety, etc., and achieve a wide range of chemical uses, short reaction time, cost low effect
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[0024] Example 1
[0025] See figure 1 The present invention provides the following technical solution: a polyphenol repairing liposome nano system comprising a liposome, polyphenol, an oxidant, a buffer having a reaction solvent, and a hydrothermal reactor having a polytetrafluoroethylene liner.
[0026] Specifically, in the present embodiment, the buffer is a PBS buffer or a Tris-HCl buffer, and the pH of the buffer is 4.0 to 10.0.
[0027] Method for preparing liposomes by polyphenol repairing liposome nano system, preparing a liposomal nano system by a pot of hydrothermal method, and specifically comprising the steps of:
[0028] Step 1: in the flowing N 2 In the environment, the phospholipids and cholesterol dissolved in the organic solvent were dried, and the sample was produced;
[0029] Step 2: The sample after drying into a lipid film was subjected to secondary drying under a vacuum environment;
[0030] Step 3: Cooling the sample to room temperature, then adding an appro...
Example Embodiment
[0038] Example 2
[0039] See figure 1 The present invention provides the following technical solution: a polyphenol repairing liposome nano system comprising a liposome, polyphenol, an oxidant, a buffer having a reaction solvent, and a hydrothermal reactor having a polytetrafluoroethylene liner.
[0040] Specifically, in the present embodiment, the buffer is a PBS buffer or a Tris-HCl buffer, and the pH of the buffer is 4.0 to 10.0.
[0041] Method for preparing liposomes by polyphenol repairing liposome nano system, preparing a liposomal nano system by a pot of hydrothermal method, and specifically comprising the steps of:
[0042] Step 1: in the flowing N 2In the environment, the phospholipids and cholesterol dissolved in the organic solvent were dried, and the sample was produced;
[0043] Step 2: The sample after drying into a lipid film was subjected to secondary drying under a vacuum environment;
[0044] Step 3: Cooling the sample to room temperature, then adding an approp...
Example Embodiment
[0052] Example 3
[0053] See figure 1 The present invention provides the following technical solution: a polyphenol repairing liposome nano system comprising a liposome, polyphenol, an oxidant, a buffer having a reaction solvent, and a hydrothermal reactor having a polytetrafluoroethylene liner.
[0054] Specifically, in the present embodiment, the buffer is a PBS buffer or a Tris-HCl buffer, and the pH of the buffer is 4.0 to 10.0.
[0055] Method for preparing liposomes by polyphenol repairing liposome nano system, preparing a liposomal nano system by a pot of hydrothermal method, and specifically comprising the steps of:
[0056] Step 1: in the flowing N 2 In the environment, the phospholipids and cholesterol dissolved in the organic solvent were dried, and the sample was produced;
[0057] Step 2: The sample after drying into a lipid film was subjected to secondary drying under a vacuum environment;
[0058] Step 3: Cooling the sample to room temperature, then adding an appro...
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