Method for extracting total chlorogenic acid from photinia fraseri

A technology of total chlorogenic acid and red leaves, which is applied in chemical instruments and methods, separation/purification of carboxylic acid esters, pharmaceutical formulations, etc., can solve the problem of high cost, and achieve the effects of increasing content, reducing loss and improving extraction effect.

Active Publication Date: 2021-11-12
武汉森澜生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Invention patent US2011/0223281 A1 discloses the weight control effect of a decaffeinated green coffee bean extract. Since green coffe

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  • Method for extracting total chlorogenic acid from photinia fraseri
  • Method for extracting total chlorogenic acid from photinia fraseri
  • Method for extracting total chlorogenic acid from photinia fraseri

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0058] Example 1 Experiment of composition analysis of red-leaf heather

[0059] Bring the harvested leaves of each stage back to the laboratory as soon as possible with clean filter paper to absorb water droplets and remove foreign bodies, cut them into pieces with scissors and weigh 5.00g in a 50ml graduated PET centrifuge tube, add 40ml of 75% ethanol / water solvent , screw the cap, and ultrasonically extract for 30min. The extract was filtered through a 0.5um organic filter, and the filtrate was injected with 2ul for HPLC analysis. The results of analysis and detection of chlorogenic acid content are as follows:

[0060] table 3.

[0061] Sample serial number Neochlorogenic acid (3-CQA) content Chlorogenic acid (5-CQA) content Total Chlorogenic Acid Content 1 1.12％ 6.02％ 7.14％ 2 1.32％ 6.74％ 8.06％ 3 2.48％ 3.56％ 6.04％ 4 3.30％ 3.48％ 6.78％ 5 2.44％ 2.84％ 5.28％ 6 1.20％ 1.46％ 2.66％ 7 1.10％ 1.04％ 2.14％ 8 0.75％ ...

Example Embodiment

[0064] Example 2 Drying Mode Screening Experiment

[0065] Harvest 67.5Kg of red-leaf heather shoots and divide them into four batches. 1 batch of bud leaves 2.5Kg is directly sun-dried; 1 batch of bud leaves 2.5Kg is directly dried in the shade; 1 batch of bud leaves is 2.0Kg, directly dried at 85°C; 1 batch of bud leaves is 60Kg, spread out to dry, use tea leaves After the greening machine is heated at 200°C, it is dried by the tea dryer at 85°C. The content of chlorogenic acid in 4 batches of dried shoots and leaves was detected. The test results are as follows:

[0066] Table 4.

[0067] batch drying method sprout leaf weight dry weight dry yield Total chlorogenic acid content in dry product 1 dry in the shade 2.5Kg 0.780Kg 31.2％ 6.8％ 2 sun dried 2.5Kg 0.755Kg 30.2％ 10.2％ 3 Direct drying at 85°C 2.0Kg 0.596Kg 29.8％ 16.8％ 4 Quick drying after finishing 60Kg 17.04Kg 28.4％ 18.4％

[0068] Experiments s...

Example Embodiment

[0069] Example 3 Preparation of the young leaf extract of red leaf heather

[0070] Using the red leaves of the red leaf heather as raw materials, prepare according to the following steps:

[0071] (1) After the red buds and leaves of the red leaf heather are killed at a high temperature of 200°C, they are quickly dried at 85°C in a tea dryer to obtain a dry product;

[0072] (2) 10kg of the dry product obtained in step (1) was pulverized, passed through a 10-mesh sieve, added with 120 liters of pure water, stirred and extracted for 60 minutes at 85±5°C, filtered through a 100-mesh stainless steel mesh, and added to 80 liters of pure water in the filter residue, After stirring at room temperature for 30min, filter through a 100-mesh stainless steel filter, and combine the secondary extraction filtrate;

[0073] (3) The combined filtrate is finely filtered by 0.3um ceramic membrane equipment (Shanghai Wodi) to remove particulate impurities, and the finely filtrated permeate is...

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Abstract

The invention discloses a method for extracting total chlorogenic acid from photinia fraseri, and belongs to the technical field of natural active matter extraction. The method takes red tender shoot leaves or red fresh leaves of photinia fraseri as a raw material, and comprises the following steps of: drying the raw material to obtain a dry product; crushing the obtained dry product, performing extraction with a solvent, and performing filtering; performing fine filtration on filtrate through a ceramic membrane; performing low-temperature concentration through an RO membrane; and performing spray drying or freeze drying on a concentrated solution to obtain a photinia fraseri extract; performing macroporous resin column adsorption and ethanol water gradient elution on a RO membrane concentrated solution to obtain an eluent; and concentrating the eluent containing the chlorogenic acid analogue through an RO membrane, and drying the concentrated solution to obtain a total chlorogenic acid active matter. Red tender shoots are selected as raw materials, and the high-content total chlorogenic acid active matter is simply and efficiently obtained from photinia fraseri leaves through a pure physical treatment process.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural active substances, and in particular relates to a method for extracting total chlorogenic acid from Photinia fruticosa leaves. Background technique [0002] Chlorogenic acid (5-O-caffeoylquinic acid, 5-CQA) is a phenylpropanoid synthesized by plant cells through the shikimic acid pathway. Substance, its isomer analog 3-CQA is neochlorogenic acid, 4-CQA is cryptochlorogenic acid. The chemical structures of chlorogenic acid and its analogues are as follows: [0003] [0004] Chlorogenic acid has various pharmacological effects such as anti-oxidation, antibacterial, anti-virus, anti-tumor, lowering blood fat, lowering blood sugar, controlling body weight and immune regulation, etc. It is widely used in the fields of food, medicine and chemical industry [Wang Qinghua et al., Research progress on the pharmacological effects and mechanisms of chlorogenic acid, Acta Pharmaceutica Sinic...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K127/00C07C67/56C07C67/48C07C69/732
CPCA61K36/73A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55C07C67/56C07C67/48C07C69/732
Inventor 邹敏亮菅保森邓思姚宏
Owner 武汉森澜生物科技有限公司
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