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Transplantation method for applying stem cells to treatment of nervous system diseases

A technique for neurological diseases and cell transplantation, applied in the field of stem cell medical research

Pending Publication Date: 2021-11-23
高谋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if a small number of differentiated stem cells are integrated in the host nervous system after transplantation, whether they can perform neural replacement functions needs further verification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The invention provides a technical solution: stem cells are used in the transplantation method for treating nervous system diseases. Through the in vitro culture experiment of animal cells, the same kind of intracranial transplantation experiment is used to directional transplant NSCs to different cerebral hemispheres of the same strain of mice. Brain Injury Restorative Therapy.

[0030] As a technical optimization scheme of the present invention, the animal cell culture experiment in vitro comprises the following steps:

[0031] S1-1: Take the telencephalon of 2 pregnant C57BL / 6 mouse embryos;

[0032] S1-2: NSCs were aseptically isolated and purified under a stereoscope and put into the culture medium;

[0033] S1-3: Take the shredded telencephalon in the ultra-clean bench, treat and digest with 0.25% trypsin and 0.02% EDTA for 5 minutes, then filter through a filter screen, filter with centrifugation, then discard the supernatant and add it to the NSCs medium for re...

Embodiment 2

[0042] The invention provides a technical solution: stem cells are used in the transplantation method for treating nervous system diseases. Through the in vitro culture experiment of animal cells, the same kind of intracranial transplantation experiment is used to directional transplant NSCs to different cerebral hemispheres of the same strain of mice. Brain Injury Restorative Therapy.

[0043] As a technical optimization scheme of the present invention, the animal cell culture experiment in vitro comprises the following steps:

[0044] S1-1: Take the telencephalon of 2 pregnant C57BL / 6 mouse embryos;

[0045] S1-2: NSCs were aseptically isolated and purified under a stereoscope and put into the culture medium;

[0046] S1-3: Take the shredded telencephalon in the ultra-clean bench, treat and digest with 0.25% trypsin and 0.02% EDTA for 5 minutes, then filter through a filter screen, filter with centrifugation, then discard the supernatant and add it to the NSCs medium for re...

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Abstract

The invention discloses a transplantation method for applying stem cells to treatment of nervous system diseases, and relates to the technical field of stem cell medical research, the transplantation method for applying stem cells to treatment of nervous system diseases comprises the following steps: directionally transplanting NSCs to different side cerebral hemispheres of mice of the same strain by adopting an isocranial transplantation experiment through an animal cell in-vitro culture experiment, and repairing and treating the brain injury of the mouse. From the influence of NSCs transplantation on mouse brain injury, by comparing transplantation injury conditions of a left intracranial transplantation experimental mouse with a high score and a right intracranial transplantation experimental mouse with a high score, left and right brain tissue structures are relatively complete, cell arrangement is regular, scar tissue formation is seldom seen, a large number of grafts are distributed along an injection needle passage, stem cell transplantation is adopted to treat a nervous system, an embryo end brain NSCs input way is selected, cells are transplanted to different side brain hemispheres of mice of the same line, and it can be guaranteed that stem cells play the maximum efficacy under the condition that neural function detection and evaluation are good.

Description

technical field [0001] The invention relates to the technical field of stem cell medical research, in particular to a stem cell transplantation method for treating nervous system diseases. Background technique [0002] The use of stem cell transplantation to treat complex nervous system diseases is the current development trend and treatment direction. [0003] Stem cells have the potential to differentiate into nerve cells. In theory, after stem cells are transplanted into the microenvironment of the nervous system, they may differentiate into neurons and glial cells, thereby exerting their functions of cell replacement and nerve repair. [0004] However, the nervous system is highly complex, and it is difficult for exogenous stem cells to integrate into the host nervous system. They generally survive in it independently for a period of time, and activate the differentiation of endogenous neural stem cells through paracrine neurotrophic factors and immune regulation, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61P25/00C12N5/0735C12N5/0797
CPCA61K35/30A61P25/00C12N5/0623C12N5/0606C12N2509/00C12N2509/10
Inventor 高谋
Owner 高谋
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