Application of lung-ventilating and toxin-vanquishing prescription to preparation of medicine for preventing and/or treating pneumonia caused by influenza A virus H1N1
An influenza virus and poison formula technology, applied in the new application field of Xuanfei Baidu formula, to achieve the effect of reducing thickening, reducing viral load, and reducing the expression of pro-inflammatory factors
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Embodiment 1
[0079] Embodiment 1 Xuanfeibaidu formula has an effect on the body weight and lungs of mice infected with influenza A (H1N1) virus
[0080] After the mice were modeled and administered in groups, the body weight changes of the mice in each group were observed for 7 days, and the results were as follows: figure 1 As shown, after 3 days of administration, the results of HE staining of mouse lung tissue in each group were as follows: figure 2 shown.
[0081] from figure 1 It can be seen from the figure that compared with the control group, the body weight of the mice in the model group decreased significantly, and compared with the model group, Xuanfei Baidu formula can significantly inhibit the weight loss of the mice infected with influenza virus.
[0082] from figure 2 It can be seen from the figure that the structure of the alveoli in the control group is complete, the shape is regular, and there is no inflammatory cell infiltration in the surrounding tissue (such as f...
Embodiment 2
[0083] Example 2 Effect of Xuanfeibaidu Recipe on the virus titer in the lungs of mice infected with influenza A H1N1 virus
[0084] The PR8-HA mRNA level in the lungs of mice infected with influenza virus was examined by RT-PCR to reflect the virus titer. Compared with the relative titer results of the control group, the lung virus titers of mice in each group were as follows: image 3 Shown (taking the relative titer of the control group as 1). from image 3 It can be seen from the figure that after 3 days of administration, compared with the control group, the virus titer in the lungs of the mice in the model group was significantly increased (P<0.001). Compared with the model group, Xuanfei Baidu formula can significantly reduce the viral load in the lungs of mice infected with influenza virus (P<0.05). It shows that Xuanfeibaidu prescription has the effect of resisting the invasion of H1N1 influenza virus and reducing the virus titer.
Embodiment 3
[0085] Example 3 Effect of Xuanfei Baidu Recipe on the Release of Inflammatory Factors in Mice Infected by Influenza A H1N1 Virus
[0086] The RT-PCR method was used to detect the changes in the mRNA levels of inflammatory factors in the lung tissue of mice infected for 3 days, and the results were as follows: Figure 4 As shown in Figure A to Figure E, it can be seen from the figure that compared with the control group, the mRNA levels of TNF-a, IL-1β, IL-6, IL-10 and Arg-1 in the lung tissue of the model group mice Significantly increased (P<0.05). Compared with the model group, Xuanfeibaidu Prescription significantly reduced the mRNA levels of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, and also increased the mRNA levels of anti-inflammatory cytokines IL-10 and Arg-1 ( P<0.05). It shows that the Xuanfei Baidu prescription of the present application can reduce the expression of pro-inflammatory factors in the lungs and promote the expression of anti-inflammatory facto...
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