Fluorescence detection system and fluorescence spectrum splicing method
A technology of fluorescence detection and fluorescence spectroscopy, which is applied in the field of laser-induced fluorescence technology and spectrum detection, can solve the problems that fluorescence acquisition does not have advantages, and the photomultiplier tube signal does not have sufficient resolution spectral information, so as to ensure accuracy and continuity, The effect of high sampling rate and high spectral wavelength resolution
Active Publication Date: 2022-02-11
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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The signal collected by a single array of photomultiplier tubes does not have sufficient resolution and sufficient spectral information, and it also does not have advantages in fluorescence collection
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[0028] In order to enable those skilled in the art to better understand the solutions of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.
[0029] In this embodiment, the system includes a dichroic film and two fluorescence detection modules as an example for detailed description. see figure 1 , which is a structural principle diagram of a fluorescent detection system provided in this implementation. A fluorescence detection system, comprising a processing module, a collimating mirror 1, a dichroic film 2 and two fluorescence detection modules, wherein the two fluorescence detection modules are respectively a first fluorescence detection module and a second fluorescence detection module; the first The fluorescence detection module includes a first slit 3, a first reflection mirror 5, a first focusing mirro...
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Abstract
The invention provides a fluorescence detection system and a method for splicing fluorescence spectra, wherein the fluorescence detection system uses a dichroic film to split light according to the wavelength, and then combines a plurality of fluorescence detection modules to extract the band of interest and splicing in Fluorescence signals are collected in the full spectrum range of visible light, and have high spectral wavelength resolution, high sampling rate, and readout rate, especially suitable for the collection and detection of weak fluorescence; and the fluorescence spectrum splicing method, the processing module can detect each fluorescence The spectral irradiance of the photomultiplier tube at the splicing part of the module spectrum is corrected, which can restore the spectral information loss caused by the dichroic film splitting, ensuring the accuracy and continuity of the spectrum collected by the system, especially suitable for the dichroic film. The case where the split wavelength is the band of interest.
Description
technical field [0001] The invention belongs to the technical fields of laser-induced fluorescence technology and spectrum detection, and in particular relates to a fluorescence detection system and a fluorescence spectrum splicing method. Background technique [0002] The fluorescence spectrometer consists of a spectroscopic device and a photodetector, and is mainly used for spectroscopic and spectral detection of fluorescent light signals. It is a basic instrument in the field of laser-induced fluorescence technology and spectral detection technology. The photodetectors commonly used in fluorescence spectrometers are CCD array photodetectors, which are characterized by extremely high spectral wavelength resolution. The detectors applied to weak fluorescent signals often use EMCCD, ICCD array photodetectors and photomultiplier tube detectors. The quantum efficiency and spatial resolution of EMCCD are high, but the time resolution is only on the order of milliseconds, which...
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IPC IPC(8): G01N21/64G01J3/44
CPCG01N21/6402G01J3/4406
Inventor 陈思颖李鑫杜宪炟黄书海陈和孙振海张寅超吴慧云郭磐
Owner BEIJING INSTITUTE OF TECHNOLOGYGY