Insecticidal Seriphidium borotalense essential oil and application thereof
A technology of essential oils and insecticides, applied in the field of botanical pesticides, can solve the problems of unreported insecticidal activity, etc., and achieve the effects of easy to obtain reagents, high pest killing rate, and simple production methods
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Embodiment 1
[0015] This example provides the preparation process and GC-MS test of the essential oil of bollotta serica.
[0016] The aerial part of the plant of Artemisia bolotta was naturally dried in the shade, crushed, weighed 500g, placed in a volatile oil extractor, and extracted by steam distillation, and the distillation time was 6h. The distillate was extracted with n-hexane to obtain the n-hexane layer, added anhydrous sodium sulfate to remove water, concentrated, measured the volume, put the volatile oil into a dry glass bottle, and stored it in a refrigerator at 4°C, which was the Boluo Tasmania essential oil.
[0017] The essential oil sample of Bolotta Serica was analyzed by GC-MS. The test conditions were: GCMS-QP2010plus (Shimadzu); Chromatographic column: DB-5MS (60m×0.25um×0.32mm) (Agilent); Heating rate: 60℃~10℃ / min-300℃-20min; split ratio: 30:1; constant linear velocity mode: 30cm / sec; carrier gas: He; ion source temperature: 200℃; interface temperature: 300℃; Temper...
Embodiment 2
[0029] This example provides the determination of the contact activity of the essential oil of Seraphim bolotta against cotton aphids and psyllids.
[0030] (1) Determination of the contact activity of the essential oil of Artemisia bolota against cotton aphids
[0031] Measured by the leaf disc method, apply polytetrafluoroethylene to the four walls of a 12-well plate, after drying, spread the agar on the bottom of the plate, take fresh cotton leaves that have not been applied with pesticides, and punch the leaves to make a diameter of 3cm The circular leaf discs were soaked in the medicine for 10 seconds, dried and put into the leaves, and then 10 gossypis aphids were inserted into them, and placed in an incubator with light / darkness=16h / 8h, 25°C, and 60%-70% humidity. No essential oil solvent and pyrethrin were used as negative and positive controls respectively. Each treatment was repeated 6 times, and 10 cotton aphids were repeated for each repetition. After 24 hours aft...
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