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Fermented feed rich in antibacterial substances and preparation method of fermented feed

A technology of fermented feed and antibacterial substances, applied in animal feed, animal feed, molding or processing of animal feed, etc., can solve the problems of complex enzyme preparation process, drug toxicity, drug residue, etc., and achieve the reduction of crude fat content and good inhibition effect, the effect of increasing the content of acid-soluble protein

Active Publication Date: 2021-12-03
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical defects that the use of antibiotics and antibacterial additives in feed will lead to drug resistance, drug residues, drug poisoning, and the addition of enzyme preparations resulting in complex processes and high costs, and provides a fermented feed rich in antibacterial substances and its Preparation

Method used

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  • Fermented feed rich in antibacterial substances and preparation method of fermented feed
  • Fermented feed rich in antibacterial substances and preparation method of fermented feed
  • Fermented feed rich in antibacterial substances and preparation method of fermented feed

Examples

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Effect test

Embodiment 1

[0041] Embodiment 1 fermentation bacteria screening

[0042]The applicant obtained a strain with the highest antibacterial activity from the soil of the mulberry field of South China Agricultural University, using the agar hole diffusion method, using Escherichia coli and Staphylococcus aureus as indicator bacteria, and obtained a strain with the highest antibacterial activity, which was designated as strain SP1.

[0043] Through the comprehensive results of morphological identification and molecular identification, it was identified that the strain SP1 belonged to Paenibacillus polymyxa, and it was preserved in the Guangdong Microbial Culture Collection Center on April 16, 2021. The address of the preservation unit is Guangdong Academy of Microbiology Research Institute (5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City), the preservation number is GDMCC No: 61605.

Embodiment 2

[0044] Embodiment 2 prepares fermented feed

[0045] 1. Weigh each component according to the following weight components:

[0046] Carbon source bran 12 parts,

[0047] Nitrogen source soybean meal 3 parts,

[0048] Dipotassium hydrogen phosphate hydrate (K 2 HPO 4 2H 2 O) 0.03 parts,

[0049] Magnesium sulfate hydrate (MgSO 4 ·7H 2 O) 0.0015 parts,

[0050] 20 parts of water,

[0051] 3 copies of Paenibacillus polymyxa, Paenibacillus polymyxa is SP1 bacteria, the density of SP1 bacteria is 1×10 6 cfu / serving.

[0052] 2. The fermented feed preparation method comprises the following steps:

[0053] S1: Raw material pretreatment: take carbon source, nitrogen source, dipotassium hydrogen phosphate hydrate, magnesium sulfate hydrate, add water and mix well; then sterilize at 120°C for 20 minutes;

[0054] S2: cultivating the SP1 bacterium to obtain a fermented liquid;

[0055] S3: feed fermentation: under aseptic environment, inoculate 3mL SP1 bacterium fermented li...

Embodiment 3

[0056] Embodiment 3 prepares fermented feed

[0057] 1. Weigh each component according to the following weight components:

[0058] Carbon source corn flour 12 parts,

[0059] 3 parts nitrogen source soybean powder,

[0060] Dipotassium hydrogen phosphate hydrate (K 2 HPO 4 2H 2 O) 0.03 parts,

[0061] Magnesium sulfate hydrate (MgSO 4 ·7H 2 O) 0.0015 parts,

[0062] 20 parts of water,

[0063] 3 copies of Paenibacillus polymyxa, Paenibacillus polymyxa is SP1 bacteria, the density of SP1 bacteria is 1×10 6 cfu / serving.

[0064] 2, the fermented feed preparation method is the same as embodiment 2.

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Abstract

The invention discloses a fermented feed rich in antibacterial substances and a preparation method of the fermented feed. The fermented feed is obtained by fermenting raw materials including a carbon source, a nitrogen source, dipotassium phosphate, magnesium sulfate, water and paenibacillus polymyxa SP1, wherein the paenibacillus polymyxa SP1 is preserved in Guangdong Microbial Culture Collection Center on April 16, 2021, and the preservation number of the paenibacillus polymyxa SP1 is GDMCC No: 61605. The preparation method of the feed disclosed by the invention is simple in process, and the fermented feed obtained by the method is rich in the antibacterial substances and can be used for replacing part of antibiotics; and meanwhile, the content of available phosphorus, protein solubility and acid-soluble proteins is increased, the content of crude fat is remarkably lowered, absorption and utilization of animals, especially young animals, are facilitated, and thus, the fermented feed has very good application and popularization values.

Description

technical field [0001] The invention relates to the technical field of fermented feed, in particular to a fermented feed rich in antibacterial substances and a preparation method thereof. Background technique [0002] In the existing breeding feed technology, antibiotics are used in large quantities, which has caused a series of problems. The use of antibiotics will increase the resistance of target microorganisms and parasites, and because of the emergence of drug resistance, people often need to further increase the dose of antibiotics when they are used again. This will intensify the emergence of drug resistance, thus forming a vicious circle and causing the abuse of antibiotics; this not only adds a lot of cost and harm to farming, but also antibiotics are difficult to disappear in a short period of time during the operation of the food chain. Human beings are at the top of the food chain Became a victim of antibiotic drug residue. Under the premise of the full implemen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K20/26A23K20/20A23K10/30A23K10/37A23K50/90A23K50/75A23K40/00
CPCA23K10/12A23K20/26A23K20/30A23K10/30A23K10/37A23K50/90A23K50/75A23K40/00Y02P60/87
Inventor 陈芳艳钟杨生李家安严会超林碧敏林健荣
Owner SOUTH CHINA AGRI UNIV
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