Endophytic fungus AM8.2 for promoting potassium absorption of camellia oleifera

A technology of AM8.2 and endophytic fungi, applied in the field of microorganisms, to achieve the effect of promoting plant growth and development, promoting plant growth, and reducing use

Active Publication Date: 2021-12-03
王晓艳 +2
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Scholars at home and abroad have screened a variety of endophytic fungi with growth-promoting properties from plants, but the current research is mainly focused on the scree...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Endophytic fungus AM8.2 for promoting potassium absorption of camellia oleifera
  • Endophytic fungus AM8.2 for promoting potassium absorption of camellia oleifera
  • Endophytic fungus AM8.2 for promoting potassium absorption of camellia oleifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation of endophytic fungi from Camellia oleifera roots

[0017] Medium:

[0018] (1) Liquid medium YPDA: 20.0g peptone, 10.0g yeast extract, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, adenine sulfate 0.03g, ultrapure water 1000ml, pH 5.6±0.2.

[0019] (2) PDA medium (potato dextrose agar medium): 6.0g potato powder, 20.0g agar, 20.0g glucose, pH 5.6±0.2.

[0020] Isolation of endophytic fungi

[0021] (1) Using the tissue separation method, the roots of camellia oleifera are rinsed with running water and then dried in an ultra-clean bench for tissue surface disinfection. The operation process is: 75vol% absolute ethanol disinfection → 2~3 times of sterile water → Disinfection with 15vol% sodium hypochlorite → wash with sterile water 2~3 times. The sterilized roots were cut off the phloem with a sterile blade and then cut into 2 mm × 2 mm in size, placed on PDA medium, and cultured at a constant temperature of 28 ° C and protected from light.

[0022] (2)...

Embodiment 2

[0032] Bacterial solution preparation: Inoculate the activated endophytic fungus AM8.2 into 20mL potato glucose liquid medium, culture on a constant temperature shaker for 72h (28°C, 220 r / min), then draw 1ml into two 500mLYPDA cultures In the medium, count the number of spores with a hemocytometer, and dilute the cultured bacterial solution with sterile water to a spore concentration of 0.6×10 9 CFU / ml.

[0033] Bacterial fertilizer preparation: The composition of the mixed solid bacterial fertilizer is: endophytic fungus AM8.2 bacterial liquid: organic fertilizer = 1 liter: 10 kg. The organic fertilizer is fermented from smoke foam, rice bran, cow dung, peanut dregs, plant ash, humic acid, sheep dung, etc., with an organic mass fraction greater than 46%, N+P 2 o 5 +K 2 O mass fraction is greater than 5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an endophytic fungus AM8.2 for promoting potassium absorption of camellia oleifera, which is screened from the root of a woody plant camellia oleifera, and belongs to the technical field of microorganisms. The endophytic fungus AM8.2 is classified and named as Colletotrichum spinosum. AM8.2, has been deposited in the China General Microbiological Culture Collection Center on September 1, 2021 with a deposit number of CGMCC No. 23245. A bacterial fertilizer prepared from the endophytic fungi AM8.2 can reduce use of chemical fertilizers, improve activities of soil microorganisms and influence soil physical and chemical properties of camellia oleifera, thereby affecting absorption of endogenous hormones and potassium and nitrogen of camellia oleifera, and promote plant growth and development.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an endophytic fungus AM8.2 that promotes the absorption of potassium in Camellia oleifera. Background technique [0002] Potassium is an essential element for crop growth and development, and the potassium content in plants generally accounts for 0.2% to 4.1% of the dry matter, which is only less than nitrogen. It can not only regulate plant metabolism, promote carbohydrate synthesis and nitrogen metabolism, but also increase crop yield, improve fruit quality, and enhance plant leaf photosynthetic performance. It can also promote the transport of photosynthetic products to tubers, and can improve the synthesis ability of sucrose in leaves. Potassium can enhance the ability of crops to resist adversity and promote the formation of good quality of crops. [0003] Camellia ( Camellia oleifera Abel. ) belongs to the Theaceae Camellia genus, and is known as the world's...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C05F11/08C05G3/00C05G3/80A01N63/30A01P21/00C12R1/645
CPCC05F11/08C05G3/00C05G3/80A01N63/30Y02W30/40
Inventor 王晓艳吴轩毅王晓伟苏军
Owner 王晓艳
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap