A kind of autologous platelet factor gel and its preparation method and application

A technology of platelet factor and platelet-poor plasma, applied in prosthesis, anticoagulation treatment, transportation and packaging, etc., can solve the problems of poor filling effect, unsatisfactory medical aesthetic effect, complex process, etc., and achieve slow absorption and excellent effect , the effect is obvious

Active Publication Date: 2022-07-01
CHENGDU QINGKE BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

The preparation of PRP by secondary centrifugation requires adding an anticoagulant (commonly used sodium citrate), which is simple to operate, high in extraction rate, wide in clinical application, and rich in application cases (osteoarthritis, etc.); however, exogenous thrombin is required for activation. Calcium ions, and the effect of medical aesthetics is not ideal, and the filling effect is poor
[0010] By continuously adjusting the rotational speed during the centrifugation process, a better gel can be obtained, the plasticity of the gel can be increased, and the membrane can be formed, etc., which can be used for osteoinductive growth, but the process is more complicated, and a specific centrifugation program needs to be established. Similarly, immediately after collection preparation, operation immediately after preparation

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  • A kind of autologous platelet factor gel and its preparation method and application
  • A kind of autologous platelet factor gel and its preparation method and application
  • A kind of autologous platelet factor gel and its preparation method and application

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[0041] The invention provides a preparation method of autologous platelet factor gel, which has flexible and unlimited time, blood collection once and multiple operations, and the obtained autologous platelet factor gel has excellent immediate effect, slow absorption, long duration of effect and safety. , convenience, and storability.

[0042] Next combine Figure 1 to Figure 2 The technical solutions of the present invention are further described with specific examples.

[0043] In embodiment 1 to embodiment 3, the specific method of collecting autologous whole blood is as follows:

[0044] Take 10-50ml of whole blood from healthy adults from the antecubital vein, store the blood in an anticoagulant tube containing EDTA, and complete the cryopreservation process within 2 hours.

[0045] The first blood sample centrifugation, 600×g, 8min, after the centrifugation is completed, suck the upper plasma into a centrifuge tube;

[0046] The second centrifugation, 1500×g, 8min, af...

Embodiment 1

[0050] The present invention provides a preparation method of autologous platelet factor gel, comprising the following steps:

[0051] Step S1, collecting autologous whole blood, first centrifuging at a speed of 600×g for 8 minutes, and after the centrifugation is completed, suck the upper plasma into a centrifuge tube; the plasma contains platelet-poor plasma (PPP) and platelet-rich plasma (PRP);

[0052] Step S2, the obtained plasma is centrifuged at a speed of 1500 × g for 8 min, the upper three-quarters of the centrifuge is taken as the upper layer, the lower quarter of the centrifuge is taken as the lower layer, and the upper layer PPP is placed in - Store at 20°C for later use, and store PRP in the lower layer at -80°C for later use;

[0053] Step S3, the upper layer liquid PPP stored in step S2 is physically modified to form a gel, and the modification method is: after freezing and thawing at -20°C, heating in a 75°C water bath for 15 minutes;

[0054] In step S4, the ...

Embodiment 2

[0061] The present invention provides a preparation method of autologous platelet factor gel, comprising the following steps:

[0062] Step S1, collecting autologous whole blood, first centrifuging at a speed of 600×g for 8 minutes, and after the centrifugation is completed, suck the upper plasma into a centrifuge tube; the plasma contains platelet-poor plasma (PPP) and platelet-rich plasma (PRP);

[0063] Step S2, the obtained plasma is centrifuged at a speed of 1500 × g for 8 min, the upper four-fifths of the centrifuge are taken as the upper layer, the lower one-fifth of the centrifuge is taken as the lower layer, and the upper layer PPP is placed in - Store at 20°C for later use, and store PRP in the lower layer at -80°C for later use;

[0064] Step S3, the upper layer liquid PPP stored in step S2 is physically modified to form a gel, and the modification method is: freezing and thawing at -20°C, and heating in a water bath at 75°C for 15 minutes;

[0065] In step S4, the...

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Abstract

The present invention provides an autologous platelet factor gel and a preparation method and application thereof. The preparation method includes the following steps: step S1, collecting autologous whole blood, firstly centrifuging to obtain plasma; the plasma includes platelet poor plasma (PPP) and platelet rich plasma Plasma (PRP); Step S2, centrifuge the obtained plasma again to obtain centrifuge, and store the upper layer liquid PPP and the lower layer liquid PRP separately for later use; Step S3, subject the obtained upper layer liquid to physical modification to form a gel; Step S4, the obtained lower layer liquid The solution is physically cracked to release factors; in step S5, the gel obtained in step S3 is mixed with the lower layer liquid after physical cracking in step S4 to obtain autologous platelet factor gel. The preparation method of the autologous platelet factor gel provided by the present invention has flexible and unlimited time, and the autologous platelet factor gel is obtained by collecting blood once and performing multiple operations, with excellent immediate effect, slow absorption, long duration of effect, safety, convenience and storability.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an autologous platelet factor gel and a preparation method and application thereof. Background technique [0002] Autologous platelet-rich plasma (PRP) is a platelet concentrate produced by the separation of autologous blood. Autologous PRP is derived from autologous and has good safety performance, which can fundamentally eliminate problems such as disease transmission and immune rejection. A large number of clinical studies have reported that PRP contains a large amount of various platelet-derived cell growth factors, which can promote the repair of various tissues. A variety of biological products are currently derived from PRP: [0003] 1. Platelet Rich Plasma (PRP): [0004] Platelet-rich plasma (PRP) is a plasma containing a high volume fraction of platelets obtained by centrifuging human or animal whole blood. The preparation of PRP by the secondary centrif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/52A61L33/00A61L33/04
CPCA61L27/3616A61L27/52A61L27/3691A61L27/3834A61L33/0041A61L33/04
Inventor 王翔王洁海泉
Owner CHENGDU QINGKE BIOTECH
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