Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pharmaceutical composition containing circ-ITCH and application thereof

A composition and drug technology, applied in the field of biomedicine, can solve problems such as the lack of effective treatment methods and the unclear molecular mechanism of cancer, and achieve great social significance and market prospects, as well as the effect of inhibiting proliferation

Inactive Publication Date: 2021-12-28
THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problems in the prior art that the molecular mechanism of the occurrence, development and metastasis of cancer, especially prostate cancer, is not clear enough and lacks effective treatment methods, so as to study the mechanism of the occurrence and development of prostate cancer. Cancer offers a new means of prevention and treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical composition containing circ-ITCH and application thereof
  • Pharmaceutical composition containing circ-ITCH and application thereof
  • Pharmaceutical composition containing circ-ITCH and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Analysis of circ-ITCH expression in prostate cancer and normal cells

[0037] (1) Collect surgically resected prostate cancer tissues and paracancerous tissues from prostate cancer patients, and sign an informed consent form with the patients;

[0038] (2) Divide each tissue sample into two parts, one part is frozen in liquid nitrogen within 30 minutes of ex vivo, and then transferred to -80°C;

[0039] (3) Use samples at -80°C for protein extraction and qRT-PCR experiments.

[0040] Experimental results such as figure 1 As shown, the results showed that compared with adjacent normal tissues, the expression of circ-ITCH in prostate cancer tissues was significantly reduced, and the difference was statistically significant (P<0.001).

[0041]Further, prostate cancer cells C4-2, Vcap, DU145, LNcap, PC3, BM-1604 and normal prostate cells RWPE-1 were selected, and the expression level of circ-ITCH was quantitatively detected by WB experiment. The results are as f...

Embodiment 2

[0043] Example 2 Proliferation and apoptosis experiments of prostate cancer cells

[0044] (1) Obtain the sequence of circ-ITCH from NCBI, its gene number is: has_circ_0059949, the position is: chr203:033091-33099197, and synthesize the sequence with enzyme cleavage sites at both ends, PacI at the 5' end and FseI at the 3' end;

[0045] (2) Obtain the circRNA overexpression plasmid, whose map is as follows image 3 As shown, the construction of the circ-ITCH overexpression plasmid was carried out, and the reaction system used to construct the plasmid is shown in Table 1 below;

[0046] Table 1 circ-ITCH overexpression plasmid reaction system

[0047] circ R Circ-ITCH dna 1ug 1ul PacI: (ul) 0.5 0.5 FseI: (ul) 0.5 0.5 10×NEBuffer(ul) 2 2 TotalRxn Volume(ul) 20 20

[0048] (3) The constructed circ-ITCH overexpression plasmid was transfected into DU145 and C4-2 cells, respectively, to construct DU145-circ-ITCH overexpression ...

Embodiment 3

[0055] Example 3 Cell scratch and invasion experiments

[0056] Select C4-2 and DU145 cells, and carry out the cell scratch experiment respectively, the specific steps are as follows:

[0057] (1) Digest the cells of the DU145-control group, DU145-Circ-ITCH group, C4-2-control group, and C4-2-Circ-ITCH group in the logarithmic growth phase, and the cell count is 2×10 5 Cells / mL were planted in a 6-well plate, and the scratch test was performed when the cell density grew to more than 95%, and serum starvation was performed for 4 hours before the experiment (if it was a serum-dependent cell, starvation was not performed);

[0058] (2) Use a 1ml pipette tip to draw lines on the vertical well plate: 2-3 lines horizontally, 2-3 lines vertically, wash the cells with 2mL PBS, a total of 3 times; replace the culture medium in the well plate with no serum culture fluid. Afterwards, culture the cells in a CO2 incubator (37°C, 5% CO2) with serum-free medium;

[0059] (3) Take photos a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a pharmaceutical composition containing circ-ITCH and application of the pharmaceutical composition to prevention and treatment of prostatic cancer. According to the composition, deep exploration is carried out aiming at related mechanisms of circular RNA in occurrence, development, treatment and prognosis of the prostate cancer, the fact that the circ-ITCH is remarkably reduced in prostate cancer cells is clear, and the reduction is related to poor prognosis of a prostate cancer patient; the total survival rate of the prostate cancer patient with relatively low circ-ITCH expression is obviously lower than that of a patient with relatively high circ-ITCH expression; and meanwhile, the circ-ITCH can remarkably inhibit proliferation, migration and invasion of prostate cancer cells in vitro. According to the composition, the effect of the cick-ITCH in the prostatic cancer is defined, a brand new thought is provided for later related drug development and clinical diagnosis and treatment, and great social significance and market prospects are achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a pharmaceutical composition containing circular RNA and its application, in particular to a pharmaceutical composition containing circ-ITCH and its application for preventing and treating prostate cancer. Background technique [0002] Prostate cancer is the most common type of cancer in the male urinary system in more than half of the countries in the world. Currently, the main treatments for prostate cancer include active surveillance, radical surgery, endocrine therapy, radiotherapy, and chemotherapy. Clinically, prostate cancer is characterized by high aggressiveness, and will eventually develop into castration-resistant prostate cancer and become resistant to current therapies, making it a hot spot and difficult point in treatment. [0003] Although the current 5-year survival of prostate cancer is constantly improving, almost all men diagnosed with prostate cancer will face sequela...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K31/7105A61P35/00
CPCC12Q1/6886A61K31/7105A61P35/00C12Q2600/158C12Q2600/178
Inventor 陈果潘斌李宇同
Owner THE FIRST AFFILIATED HOSPITAL OF JINAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com