Anaerobic ammonium oxidation bacteria particle culture method and device

An anammox bacteria and culture method technology, applied in the field of environmental protection microorganisms, can solve the problems of easy bonding of fiber fillers into agglomerates, inability to achieve a mixed state, poor solid-liquid contact effect, etc. Expand training and reduce the effect of manual intervention

Pending Publication Date: 2021-12-31
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The particle size of the powder provided by this method is 10-50μm, and the density is 1.5-2.5g / cm3. The powder particles are small, and the density is higher than that of water, and it is easy to settle. Therefore, it will be stratified with the sludge with low density during the cultivation process. achieve a homogeneous mix
Furthermore, Chinese patent CN10593685A discloses a method for enriching and cultivating anammox bacteria by using modified basalt fiber as a suspension filler and mixing it with sludge, but in this method, the fiber filler is easily bonded into agglomerates, resulting in a decrease in specific surface area The effect of contact with solid and liquid is not good, so the processing efficiency is not high

Method used

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  • Anaerobic ammonium oxidation bacteria particle culture method and device
  • Anaerobic ammonium oxidation bacteria particle culture method and device
  • Anaerobic ammonium oxidation bacteria particle culture method and device

Examples

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Embodiment approach

[0031] see Figure 1-2 , as a typical embodiment of the present invention, a kind of anammox bacterial granule culture device, comprises enrichment nutrient solution tank 1, upflow anaerobic reactor 3, first water pump 2, medium storage tank 4, the second Two water pumps 5 and the denitrification reactor 6, the upflow anaerobic reactor 3, the middle storage tank 4 and the denitrification reactor 6 are connected in sequence; The oxygen reactor 3 is connected through the first water pump 2, and the top of the upflow reactor 3 is provided with a three-phase separator 301, and the three-phase separator 301 is provided with a liquid outlet, and the liquid outlet is connected to the middle through a pipeline. The storage tank 4 is connected, and the middle storage tank 4 is connected with the denitrification reactor 6 through the second water pump 5. The top of the denitrification reactor 6 is provided with a gas outlet, and the gas outlet is connected to the upflow type via a gas p...

Embodiment 1

[0038] A method for cultivating anaerobic ammonium oxidizing bacteria granules, comprising the steps of:

[0039] S1. Using domestic sewage as the influent, mix granular fillers and nitrification sludge for intermittent aeration culture, set the aeration temperature to 20°C, and the dissolved oxygen concentration to 2 mg / L, and stop the aeration after 6 hours of air aeration. Place the sediment for 0.5h, discharge the supernatant after standing, add the same volume of water and let stand for 3h, this is an aeration culture cycle; repeat the culture cycle to more than 20 days to complete the intermittent aeration culture of granular fillers;

[0040] S2. Fill the granular filler obtained in step S1 after intermittent aeration culture into the hollow carrier on the mesh surface as a film-hanging filler layer and place it in the anaerobic reactor, put in nitrification sludge, and feed the enriched nutrient solution to start the anaerobic reactor. Oxygen ammonium oxidation culture...

Embodiment 2

[0050] A method for cultivating anaerobic ammonium oxidizing bacteria granules, comprising the steps of:

[0051] S1. Using domestic sewage as the influent, mix granular fillers and nitrifying sludge for intermittent aeration culture, set the aeration temperature to 30°C, and the dissolved oxygen concentration to 3 mg / L, and stop the aeration after 7 hours of air aeration. Place the sediment for 1 hour, discharge the supernatant after standing, add the same volume of water and let stand for 4 hours, this is an aeration culture cycle; repeat the culture cycle to 20 days to complete the intermittent aeration culture of granular fillers;

[0052] S2. Fill the granular filler obtained in step S1 after intermittent aeration culture into the hollow carrier on the mesh surface as a film-hanging filler layer and place it in the anaerobic reactor, put in nitrification sludge, and feed the enriched nutrient solution to start the anaerobic reactor. Oxygen ammonium oxidation culture;

[...

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Abstract

The invention particularly relates to an anaerobic ammonium oxidation bacteria particle culture method and device. The culture method comprises the following steps: firstly, taking domestic sewage as inlet water, mixing a particle filler with nitrification sludge, carrying out intermittent aeration culture, placing the obtained granular filler in a net-surface hollow carrier as a filler layer, filling the filler layer in an anaerobic reactor, performing anaerobic ammonia oxidation culture, and applying a nitrogen source generated by a denitrification process to an anaerobic ammonia oxidation process to realize enrichment of anaerobic ammonia oxidation bacteria particles. The culture method disclosed by the invention has the characteristics of rapid anaerobic ammonium oxidation starting, high thallus concentration and short culture period, the granular filler is fixed in the reactor to serve as a filler layer, the acceleration of starting and rapid mass propagation of anaerobic ammonium oxidation bacteria is facilitated, and the device is high in treatment efficiency, stable in operation and low in energy consumption.

Description

technical field [0001] The invention relates to the field of environment-friendly microorganisms, in particular to a method and device for cultivating anaerobic ammonia oxidizing bacteria granules. Background technique [0002] The domestic sewage generated in life, or the production sewage produced in food, slaughtering, breeding, circuit board and other industries often contain a large amount of ammonia nitrogen. If these ammonia nitrogen are not treated properly, they will easily cause eutrophication in the water body if they are discharged into natural water bodies. change. At present, the emission standards of ammonia nitrogen and total nitrogen have been clearly defined, and the water quality required for surface water treatment is getting higher and higher. Therefore, harmless treatment of nitrogen produced in daily life and production to realize a virtuous cycle of nitrogen is a necessary measure to realize the harmonious coexistence between man and nature. [0003...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F3/34C02F101/30
CPCC02F3/286C02F3/34C02F2003/001C02F2101/30C02F2305/06Y02W10/10
Inventor 李志弘谭平洋张保安杨秋婵
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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