Strain character maintaining method of strain for edible mushroom production

A technology for edible fungi and strains, which can be applied in the methods of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as strain degradation, and achieve the effect of shortening bacterial age, excellent production performance, and improved synchronization.

Active Publication Date: 2021-12-31
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of strain degradation of the current edible fungus production strains after many years or large-scale production, and adopt tissue separation combined with single hyphae purification method to develop a rejuvenating strain suitable for the actual industrial development needs of edible fungi production, providing a better solution to strain degradation

Method used

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  • Strain character maintaining method of strain for edible mushroom production
  • Strain character maintaining method of strain for edible mushroom production
  • Strain character maintaining method of strain for edible mushroom production

Examples

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Embodiment 1

[0030] In 2019, the method of the present invention was utilized to carry out the species maintenance of the production strain of No. 1 Mushroom Nongxiang, and the steps were as follows:

[0031] (1) The mushroom cultivation and spores are collected on the insect and disease-free fungus packs in the factory area. Three fruiting bodies with typical species characteristics and healthy growth are selected and retained, and the culture is continued until the bacterial membrane of the fruiting bodies is completely ruptured, and the caps are collected. And rinse the surface with 75% alcohol solution to disinfect 2-3 times, and place it in a self-made spore collector to collect spores ( figure 1 ).

[0032] (2) Single spore isolation and culture Select spore prints with complete spore print edges and clear gill traces ( figure 2 ), using an inoculation ring sterilized by flame burning in advance, scraping an appropriate amount of spores in the middle of the gill trace along the dir...

Embodiment 2

[0041] In 2019, the method of the present invention was used to carry out the species maintenance of the strain for production of Hericium erinaceus, an excellent variety of Hericium erinaceus, and the specific screening steps were the same as in Example 1. The hybrid progeny strain HT12 with the typical species characteristics of the original strain was screened out, and the original strain was used as a reference. Regional test and production test of Hericium 96 progeny strain HT12.

[0042] The results showed that the fruiting body of Jin Hericium 96 progeny strain HT12 obtained by this method was white when fresh, sessile, solitary, 10 cm-13 cm in diameter, and the mushroom body was light yellow after drying, soft and elastic, and not easy to crack. . Compared with the control original strain, the hyphae in the progeny strain HT12 were more dense, and the roundness of fruit bodies, fruiting uniformity, mushroom body density and yield were significantly improved, especiall...

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Abstract

The invention discloses a strain character maintaining method of a strain for edible mushroom production. The strain character maintaining method comprises the following steps: culturing seed mushrooms, collecting spores, separating monospores, identifying monokaryotic strains with different mating types, acquiring a group of monokaryotic strains with the same mating type as an original strain, acquiring strains for production with typical strain characters of the original strain, and screening strains for production with a production performance superior to that of the original strain. Compared with the prior art, The method fully utilizes characteristics of sexual offspring inheritance of edible mushrooms and obtains offspring strains with dominant biological and economic characters of the original strain. The offspring strains obtained by the method have the characters of the original strain and a superior production performance than the original strain. The method can effectively shorten a mushroom age, improves synchronism of spawn running and fruiting, and effectively avoids industrial problems of yield reduction, quality decline and even no production caused by over-old mushroom age due to long-time and large-scale use of the production strain.

Description

technical field [0001] The invention relates to the field of production of edible fungus strains, in particular to a method for screening and maintaining the species of edible fungus progeny strains whose hyphae of production strains can produce a lock-like combination. Background technique [0002] my country is the world's largest producer and consumer of edible fungi. The total output of edible fungi products accounts for about 70% of the world's total output. It is also the fifth largest modern agriculture in my country after grain, vegetables, fruit and oil. leading industry. With the development of economy and society, edible fungi have been gradually recognized and accepted by people due to the complete body of essential amino acids and the rich content of functional active substances. [0003] At present, my country's edible fungus production enterprises mostly use asexual reproduction methods such as mycelium propagation and fruit body separation to breed bacteria. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12N1/02C12Q1/04C12R1/645
CPCC12N1/14C12N3/00C12N1/02C12Q1/04
Inventor 刘靖宇常明昌张敏孟俊龙邓冰袁学文
Owner SHANXI AGRI UNIV
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