Integrated detection method for safrole and dihydrosafrole in ginger and ginger powder

Abstract

The invention discloses an integrated detection method for safrole and dihydrosafrole in ginger and ginger powder, and belongs to the technical field of pesticide residue detection. The method comprises the steps: preparing a mixed standard solution of safrole and dihydrosafrole; adding ginger into a methanol solution, and adding the mixed standard solution to obtain a ginger sample to be detected; adding the ginger powder into a headspace bottle, adding NaCl and a mixed standard solution, and performing extracting to obtain a ginger powder sample to be detected; and then performing GC-MS / MS detection on the ginger sample to be detected and the ginger powder sample to be detected to obtain the content of safrole and dihydrosafrole in the ginger and the ginger powder. By means of an integrated sample headspace solid-phase microextraction and gas chromatography-mass spectrometry instrument and program setting, high selectivity, high accuracy and repeatability of results can be achieved through sample solid-phase microextraction and gas chromatography-mass spectrometry integrated detection, few solvents are used in the whole process, operation is easy and convenient, and labor cost is greatly saved.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to an integrated detection method for safrole and dihydrosafrole in ginger and ginger powder. Background technique [0002] Safrole, also known as safrole and safrole, is a plant toxin that naturally exists in natural aromatic plants such as ginger and cinnamon and camphor plants. The main component of camphor oil. Safrole aroma substances belong to carcinogenic alkylbenzene compounds. In recent years, studies have shown that safrole can form safrole-deoxyribonucleic acid (DNA) adducts in liver tissue. When the content of safrole in food is more than 0.5%, it will cause Hepatoma has certain toxic and side effects on the human body, and can easily induce gene mutations, liver damage, and even liver cancer. It is a strong carcinogen of the digestive system, blood system, and urinary system. Ingestion of sufficient doses of safrole can cause oxidative ...

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Problems solved by technology

[0004] At present, the analysis methods of safrole compounds in the literature focus on the detection of instrumental analysis. The pretreatment is mainly to add solvent extraction to the sample, and take the extract into the instrument for analysis. However, the pretreatment takes a long time and consumes a lot of solvent. Some methods need to use Purification of solid-phase extraction cartridges takes a long time, is costly, and pollutes the environment, and the solvent is volatile, which will lead to changes in the determination results of the target

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