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Method for detecting 12 active ingredients in honeysuckle medicinal material

An active ingredient, the technology of honeysuckle, applied in the field of biotechnology detection, can solve the problems of cumbersome detection process, limited number of ingredients, and inability to quantify a single phenolic acid alone

Pending Publication Date: 2022-01-11
上海中科新生命生物科技有限公司
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Problems solved by technology

The number of components determined by this method is limited, and single phenolic acids (such as isochlorogenic acids A, B, C) cannot be quantified separately, and the detection sensitivity is low, the detection process is cumbersome, and it is not suitable for large-scale production practice among

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  • Method for detecting 12 active ingredients in honeysuckle medicinal material
  • Method for detecting 12 active ingredients in honeysuckle medicinal material
  • Method for detecting 12 active ingredients in honeysuckle medicinal material

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Embodiment Construction

[0033] The technical solutions of the present invention will be further elaborated below in conjunction with specific embodiments and accompanying drawings, and these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0034] Main experimental instruments and reagents:

[0035] laboratory apparatus:

[0036] Chromatographic instrument ACQUITY UPLC I-Class (Waters); 5500QTRAP mass spectrometer (SCIEX); chromatographic column is Waters HSS T3, model 2.1×100mm, 1.8μm, catalog number 186003539; Vanquish Flex UHPLC ultra-high performance liquid chromatography (Thermo Scientific) ; Q-Exactive HF-X series mass spectrometer (Thermo Scientific); the chromatographic column is Waters HSS T3, the model is 2.1 × 100mm, 1.8μm, item number 186003539; low temperature high-speed centrifuge (Eppendorf 5430R):

[0037] List of reagents used in the experiment:

[0038]

[0039] A method for detecting 12 kinds of acti...

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Abstract

The invention provides a method for detecting 12 active ingredients in a honeysuckle medicinal material, and belongs to the technical field of biological detection. A liquid chromatography-mass spectrometry technical means is adopted for detection, and the detection result of a honeysuckle traditional Chinese medicine extract sample is identified in a spectrogram comparison mode, 12 important active substances in the honeysuckle traditional Chinese medicine extract are accurately identified, the relative content of the 12 important active substances is further calculated, and the method has the advantages of high precision, simple pretreatment, high sensitivity, good repeatability and the like, and has very important guiding significance on efficacy evaluation and production practice of honeysuckle.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, and in particular relates to a method for quantitatively analyzing 12 effective components in honeysuckle medicinal materials by using liquid chromatography mass spectrometry technology. Background technique [0002] Honeysuckle (Lonicera japonica), also known as honeysuckle. The name "Honeysuckle" comes from "Compendium of Materia Medica". Because the honeysuckle flower is white at first, and then turns yellow, it is named honeysuckle. From the emergence of "Atypical Pneumonia" (SARS) in 2002 to "New Coronary Pneumonia" in 2020, the combination of traditional Chinese and Western medicine has become more and more mature in the treatment of this sudden pathogenic bacteria, and antiviral and antibacterial Chinese medicinal materials have also played a role. It has a significant curative effect, and one of the representative medicines is honeysuckle. Pharmacological studies have shown that o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 阮宏强刘泰驿张惠萍
Owner 上海中科新生命生物科技有限公司
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