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Potassium ion transporter protein HbRAR1 and application thereof in regulation and control of potassium transport of plants

A potassium ion and plant technology, which is applied to the potassium ion transporter protein HbRSAR1 and its application in regulating the potassium transport of plants, can solve the problems such as the regulation mechanism that has yet to be explored and analyzed.

Active Publication Date: 2022-01-14
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

These research results confirm that the signaling pathway of potassium and the signaling pathway of lateral root development are intersected, but the core and key regulatory mechanism is still to be discovered and analyzed

Method used

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  • Potassium ion transporter protein HbRAR1 and application thereof in regulation and control of potassium transport of plants
  • Potassium ion transporter protein HbRAR1 and application thereof in regulation and control of potassium transport of plants
  • Potassium ion transporter protein HbRAR1 and application thereof in regulation and control of potassium transport of plants

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Embodiment 1

[0083] Materials and Methods:

[0084] Cultivation of wild barley materials: Salt pasture wild barley was collected from the saline grassland of Inner Mongolia (August 2006, Beijing Academy of Agriculture and Forestry Sciences collected from the suburb of Hohhot, Inner Mongolia, contact information: Beijing Academy of Agriculture and Forestry Sciences, No. 9, Shuguang Huayuan Middle Road, Haidian District, Beijing Li Ruifen 15811108560).

[0085] After wild barley seeds were soaked in deionized water for 12 hours at room temperature, they were placed in a refrigerator at 4°C overnight. Rinse three times with sterilized deionized water, put them in a culture dish with wet gauze, and germinate at 25°C. After 4 to 5 days after most of them germinate, transfer them to a sterilized glass bottle filled with 1 / 2 Hoagland's culture solution (the glass bottle is wrapped with opaque paper), at a temperature of 22-23°C, under light Strength 1000-3000μmol m -2 the s -1 , grow under th...

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Abstract

The invention discloses a high-efficiency potassium ion transporter HbRAR1 separated and identified from saline-grown wild barley as well as a related biological material and application of the high-efficiency potassium ion transporter HbRAR1. Transgenic experiments of introducing a gene coding the potassium ion transporter protein HbRAR1 into arabidopsis thaliana, brachypodium distachyon and potassium ion transport deficient yeast Cy162 prove that the potassium ion transport efficiency in an adversity environment, especially in a salt stress environment, is remarkably promoted by overexpression of the related gene, and meanwhile, the growth and development of plant roots under various stresses are promoted, comprising root hair elongation and lateral root growth. Therefore, the potassium ion transporter protein HbRAR1 is related to transport of potassium ions, can be used for promoting absorption and utilization of potassium by plants and increasing the yield of the plants.

Description

technical field [0001] The invention relates to the potassium ion transporter protein HbRSAR1 in the field of biotechnology and its application in regulating the transport of potassium by plants. Background technique [0002] The stress resistance of plants has always been a bottleneck in the development of modern agriculture. Using special stress-resistant germplasm resources to excavate unique stress-resistant mechanisms has extraordinary significance for improving the environment, increasing land utilization and crop yields. Halophytic wild barley (Hordeum brevisubulatum (Trin.) Link) is a perennial halophyte of the genus Poaceae Hordeum, with strong adaptability, especially able to grow well on severe saline-alkali land with a salt content of 0.6-1.0%, showing very strong salt-tolerant properties. Salt-grown wild barley is not only an excellent pasture, but also a pioneer plant for improving saline-alkali land. At the same time, as a wild relative of barley and wheat, i...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10A01H5/00A01H6/20
CPCC07K14/415C12N15/8273C12N15/8271Y02A40/10
Inventor 张海纹李瑞芬江颖冯浩
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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