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Method for preventing and controlling soil antibiotic resistance gene pollution

A technology of antibiotic resistance and resistance gene, which is applied in the restoration and fermentation of contaminated soil, can solve the problems of preventing and controlling the spread of antibiotic resistance genes, lack of microbial community regulation, etc., and achieve green environmental protection prices, easy storage and The effect of transportation, broad application prospects

Pending Publication Date: 2022-01-18
ZHEJIANG GONGSHANG UNIVERSITY +1
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] These methods all point out the methods to remove complex pollutants such as heavy metals, antibiotics, and resistance genes in the soil environment, but generally lack the regulation of microbial communities in complex polluted soils. Studies have shown that the composition of bacterial communities is an important factor for antibiotics in soil. The main determinant of resistance gene content, but the existing patent does not involve the use of antibiotic resistance gene inhibitors to select host microorganisms for antibiotic resistance genes to prevent the spread of antibiotic resistance genes

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  • Method for preventing and controlling soil antibiotic resistance gene pollution
  • Method for preventing and controlling soil antibiotic resistance gene pollution
  • Method for preventing and controlling soil antibiotic resistance gene pollution

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preparation example Construction

[0040] The present application found that agricultural enzymes can effectively inhibit the increase of actinomycetes in the soil caused by the application of pig manure organic fertilizer, and reduce the proliferation and spread of antibiotic resistance genes in the soil through the selection of host microorganisms. The preparation method of agricultural enzyme comprises:

[0041] (1) Collect 3-6 parts by weight of food waste and grind them to obtain a food mixture; the food waste is preferably vegetable and fruit waste, such as discarded vegetable leaves, fruit peels, pea shells, etc.

[0042] (2) Add 10-20 parts by weight of water and 1-2 parts by weight of brown sugar into the fermenter and fully stir to dissolve the brown sugar completely to obtain a brown sugar solution;

[0043](3) Pour the food waste in step (1) into the fermenter, add 1-2 parts by weight of fermentation powder and stir until fully mixed to obtain a slurry, then seal it, make a label and paste it on the...

Embodiment 1

[0050] The preparation of embodiment 1 agricultural ferment

[0051] The ratio of raw materials is shown in Table 1:

[0052] Table 1 raw material formula (weight part)

[0053] serial number food waste water brown sugar baking powder 1# 5 15 1.5 1.5 2# 6 20 2 2 3# 3 10 1 1

[0054] According to the formula in Table 1, the operation steps are as follows:

[0055] (1) Collect the food wastes of formula quantity (including discarding vegetable leaves, fruit peels, pea shells, etc.) and grind them to obtain food mixture;

[0056] (2) add the water of formula quantity and brown sugar in fermenter and fully stir, make brown sugar dissolve completely, obtain brown sugar solution;

[0057] (3) Pour the food waste in step (1) into the fermentation barrel, add the formula amount of fermentation powder and stir until fully mixed to obtain a slurry, then seal it, make a label and paste it on the barrel, and place it in a ventilated and sha...

Embodiment 2

[0061] Example 2 The experiment of using agricultural enzymes to inhibit and control the resistance genes of β-lactams and fluoroquinolones in the soil. The experimental site: the experimental field of Yangdu Scientific Research Base in Haining, Zhejiang Province.

[0062] The original abundance of β-lactam and fluoroquinolone resistance genes in the soil were 84.69±19.17 and 106.78±26.78ppm, respectively. Subsequently, the crop tomato was planted, and conventional water and fertilizer management was adopted. The fertilizer applied was Zhejiang Hangzhou Xiaoshan Organic Fertilizer Co., Ltd. Pig manure organic fertilizer, the 1# agricultural enzyme in embodiment 1 is diluted 10 times with water to get diluted 10 times ferment, and every plant tomato root soil applies 15ml gained dilute 10 times ferment after planting begins. After the tomato is mature, take the soil at 2-10cm from the tomato root for detection and analysis. Pig manure organic fertilizer was applied alone as a c...

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Abstract

The invention discloses a method for preventing and controlling soil antibiotic resistance gene pollution; an agricultural enzyme is used as an impedance agent to be directly applied to polluted soil to be treated or applied to the soil to be treated after fertilizer is applied or applied to the soil to be treated after being evenly mixed with the fertilizer; the agricultural enzyme is a liquid fermentation product obtained by fermenting food wastes; and the polluted soil to be treated is soil polluted by antibiotic resistance genes taking actinomycetes as host bacteria. According to the method, proliferation and diffusion of antibiotic resistance genes in soil are reduced by means of inhibition of the agricultural enzyme on survival of microorganisms such as actinomycetes, namely selection of host microorganisms; and the method is an efficient, broad-spectrum, green, environment-friendly and low-price agricultural environment restoration technology.

Description

technical field [0001] The invention belongs to the field of environmental resistance gene pollution control, and specifically relates to a method for alleviating soil resistance gene pollution caused by organic fertilizer application by changing soil microorganisms Background technique [0002] The increase in the abundance of antibiotic resistance genes can reduce the efficacy of antibiotic drugs, which is the root cause of antibiotic resistance in bacteria and is an increasingly serious global public health problem. Farmland soil is an important source and sink of antibiotic resistance genes, and its research has attracted extensive attention. Due to the existence of host microorganisms rich in antibiotic resistance genes in organic fertilizers, and the persistent selection pressure of antibiotics and heavy metals, their use has made an important contribution to the increase in the abundance of antibiotic resistance genes in farmland soil. Data show that compared with 19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00B09C1/10
CPCC12P1/00B09C1/10B09C2101/00
Inventor 汪美贞林达姚燕来王卫平陈再明张璟晗连雨露
Owner ZHEJIANG GONGSHANG UNIVERSITY
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