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Digital micro-fluidic chip device for nucleic acid detection and use method

A microfluidic chip and microfluidic channel technology, applied in the field of medical testing and analysis, can solve problems such as not being widely used, complex manufacturing, etc., achieve good clinical and commercial value, reduce demand, and increase the effect of analyzing sample volume

Pending Publication Date: 2022-01-25
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although great progress has been made in cdPCR research, they still require complex fabrication, as well as additional control components such as syringe pumps, air pressure, control systems, etc., and thus are not widely used

Method used

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  • Digital micro-fluidic chip device for nucleic acid detection and use method
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  • Digital micro-fluidic chip device for nucleic acid detection and use method

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Embodiment Construction

[0039] Embodiments of the invention are described in detail below, examples of which are illustrated in the accompanying drawings. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0040] like figure 1 As shown, a digital microfluidic chip device for nucleic acid detection includes an upper substrate and a lower substrate with a microfluidic channel sealed with the upper substrate; the microfluidic channel in the lower substrate is composed of Composed of sample inlet, array chamber, water storage tank and sample outlet; the upper substrate is made of glass with a size of 25x76mm; the lower bottom plate is made of polydimethylsiloxane, cycloolefin copolymer or cycloolefin polymer , the size is 23x50mm; the depth of the array chamber provided on the lower bottom plate is 10-200 microns, the width of the micro-pipe is 1-10 mm; the width of the micro...

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Abstract

The invention discloses a digital micro-fluidic chip device for nucleic acid detection and a use method. A nucleic acid detection aqueous phase solution and an oil solution with the specific gravity smaller than that of water are added to a chip sample inlet at a time, then a chip is fixed on centrifugal equipment, and a water phase and an oil phase can be driven to enter the chip through centrifugation for 10-20 minutes. Finally, the result that the water phase is distributed in a micro-cavity of the chip and the oil phase is distributed in a micro-pipeline is obtained. Only 1-3 nanoliters of volume of reagent is needed for chip analysis of the invention. The analysis cost is greatly reduced, and the requirement on the sample content is reduced. The water phase and the oil phase can be injected into the chip at the same time, impurities are prevented from being introduced in multi-step experiments, and the accuracy of analysis is improved. The chip is simple in design principle, and samples can be separated by a one-step method only through a centrifugal device, so that digital PCR detection is realized.

Description

technical field [0001] The invention relates to a digital microfluidic chip device for nucleic acid detection and a method for using it, belonging to the field of medical inspection and analysis. Background technique [0002] Liquid biopsies have grown significantly in recent years due to their minimal invasiveness and potential to assess biomarkers of various diseases. It includes circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), cell-free DNA (cfDNA), extracellular vesicles (EVs), etc. Plasma is a common carrier for liquid biopsy and is a dynamic medium in which circulating nucleic acids can be shed from cells and released into the blood. Thus, circulating nucleic acids offer unique opportunities for diagnosis or monitoring of cancer dynamics. [0003] Nucleic acids released by tumor cells make up a small fraction of plasma, therefore, highly precise and robust methods are required to detect and quantify small numbers of nucleic acid molecules. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/24C12M1/34C12M1/38C12Q1/6837
CPCB01L3/5027C12Q1/6837
Inventor 秦玉岭胡文琪唐曲陈欢欢毛天智
Owner NANTONG UNIVERSITY
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