Depression modeling box and modeling method

Abstract

The invention relates to the technical field of animal experiment devices, in particular to a depression modeling box and a modeling method. The depression modeling box comprises a box body and a transparent partition plate, the transparent partition plate is in a T shape, the transparent partition plate is arranged in the box body and divides the inner space of the box body into three independent subspaces including the first subspace, the second subspace and the third subspace, the cross section area of the first subspace is larger than that of the second subspace and that of the third subspace, the cross section area of the second subspace is equal to that of the third subspace, and a first through hole communicated with the first subspace, a second through hole communicated with the second subspace and a third through hole communicated with the third subspace are formed in the side wall of the box body. The depression modeling box can contain a rat and two mice at the same time, the rat serves as a predator pressure source to continuously give pressure stimulation to the mice, depression behaviors of the mice are caused, the stimulation intensity is high, and the modeling success rate and the modeling efficiency are high.

Description

technical field [0001] The invention relates to the technical field of animal experiment devices, in particular to a depression modeling box and a modeling method. Background technique [0002] The instinctive fear response of animals is inherent, and a moderate instinctive fear response (such as the animal fleeing, staying still, or starting a fighting state in a crisis situation) is extremely important for the survival and reproduction of animals. However, abnormal instinctive fear reactions are closely related to the occurrence of depression and other mental diseases. Instinctive fear response is a very ancient and conservative behavior in evolution, which determines that it must be regulated by conservative brain circuits. In modern life, human beings no longer need to deal with the threat of natural enemies, but instead use it to deal with the "imaginary enemy" in the spirit. For example, patients with depression often show excessive responses to negative stimuli, and...

AI Technical Summary

Problems solved by technology

2) Chronic unpredictable stress model

Among these two modeling methods, the first method is difficult to effectively model depression in female mice, and social frustration can only represent a part of depression in patients with social problems leading to depression, which lacks universality

The second method, chronic unpredictable model, takes a long time to model, the experimental process is complicated, and there is also the problem of limited success rate of female mice.

[0004] At present, there is still a modeling method (described in the article by Jillybeth Burgado et al. in 2014), that is, using rats as a source of predator stress to give mice stress stimulation, causing depression-like behavior in mice, but the problem with this scheme is: the Protocol for 15 days of modeling. Mice were protected daily in hamster balls and then placed in the rat area for 30 minutes. This stimulation failed to provide sustained rat stress and the mice had no direct contact with predators. Stimulus intensity is weak

Images