Micro-nano hydrogel microsphere for targeted adjustment and control of mitochondrial respiratory chain as well as preparation and application of micro-nano hydrogel microsphere

A technology of hydrogel microspheres and mitochondria is applied in the field of biomedical engineering materials to achieve the effects of protecting mitochondria, promoting production and improving function

Active Publication Date: 2022-02-15
川北医学院附属医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the joint cavity of OA patients, because the inflammatory cells of the cartilage secrete a variety of inflammatory factors and metalloproteinases (MMPs), the synovial tissue is in a state of inflammation and sw...

Method used

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  • Micro-nano hydrogel microsphere for targeted adjustment and control of mitochondrial respiratory chain as well as preparation and application of micro-nano hydrogel microsphere
  • Micro-nano hydrogel microsphere for targeted adjustment and control of mitochondrial respiratory chain as well as preparation and application of micro-nano hydrogel microsphere
  • Micro-nano hydrogel microsphere for targeted adjustment and control of mitochondrial respiratory chain as well as preparation and application of micro-nano hydrogel microsphere

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Embodiment Construction

[0039] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be described in detail below in conjunction with the examples. It must be pointed out that the following examples are only used to explain and illustrate the present invention, and are not intended to limit the present invention . Some non-essential improvements and adjustments made by those skilled in the art based on the above content of the invention still belong to the protection scope of the present invention.

[0040] 1. Examples of implementation methods

[0041] 1 Materials and methods

[0042] 1.1 Main reagents:

[0043] The SS-31 peptide was provided by Selleck China (Shanghai Bluewood Chemical Co., Ltd.); the Wyrgrl peptide was provided by China Tricaptide; DSPE-PEG 5000 -NH 2 (Distearoylphosphatidylethanolamine-N-amino-polyethylene glycol 5000), DSPE-PEG 2000 -NHS (Distearoyl Phosphatidylethanolamine-Polyethylene Glycol 2000-S...

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Abstract

The invention provides a micro-nano hydrogel microsphere for targeted adjustment of a mitochondrial respiratory chain as well as preparation and application of the micro-nano hydrogel microsphere. According to the invention, hyaluronic acid microspheres are prepared through a microfluidic technology, and SS-31 peptide and Wyrgrl peptide modified resveratrol-loaded long-circulating liposome are connected in a microsphere nano network through a non-covalent bond, so that a micro-nano hydrogel microsphere system with a targeted cell MRC adjustment function is constructed. The system has efficient cellular uptake efficiency and mitochondrial targeting, and can significantly improve the MRC function, reduce proton leakage, protect mitochondria, down-regulate ROS expression, and promote generation of cartilage extracellular matrix. Meanwhile, the system can effectively slow down the progress of osteoarthritis in a rat osteoarthritis model. The micro-nano hydrogel microsphere system for the targeted adjustment and control of the cell MRC function, provided by the invention, has great potential in treating various degenerative diseases related to MRC dysfunction.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering materials and relates to a hydrogel microsphere, in particular to a micro-nano hydrogel microsphere for targeting and regulating the mitochondrial respiratory chain and its preparation and application. Background technique [0002] Mitochondria are important organelles in eukaryotic cells. They regulate energy metabolism and cell cycle through multiple pathways such as apoptosis and autophagy. They are the "energy factories" of eukaryotic cells. The mitochondrial respiratory chain (MRC) is a process in which electron transport (ETC) is coupled with adenosine triphosphate (ATP) synthesis, which takes place in the inner mitochondrial membrane (IMM). When cells are affected by multiple factors such as aging and inflammation, electron leakage occurs in the electron transfer of the mitochondrial respiratory chain, and the leaked electrons directly form oxygen free radicals with oxygen mo...

Claims

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Application Information

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IPC IPC(8): A61K38/07A61K31/05A61K47/60A61K47/62A61K47/69A61K9/06A61K47/36A61P29/00A61P29/02A61P19/04
CPCA61K38/07A61K31/05A61K47/60A61K47/62A61K47/6911A61K9/06A61K47/36A61P29/00A61P29/02A61P19/04A61K2300/00Y02P20/55
Inventor 李毓灵张静王星宽崔文国蒋科向超严才平
Owner 川北医学院附属医院
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