Micro-nano hydrogel microsphere for targeted adjustment and control of mitochondrial respiratory chain as well as preparation and application of micro-nano hydrogel microsphere

Abstract

The invention provides a micro-nano hydrogel microsphere for targeted adjustment of a mitochondrial respiratory chain as well as preparation and application of the micro-nano hydrogel microsphere. According to the invention, hyaluronic acid microspheres are prepared through a microfluidic technology, and SS-31 peptide and Wyrgrl peptide modified resveratrol-loaded long-circulating liposome are connected in a microsphere nano network through a non-covalent bond, so that a micro-nano hydrogel microsphere system with a targeted cell MRC adjustment function is constructed. The system has efficient cellular uptake efficiency and mitochondrial targeting, and can significantly improve the MRC function, reduce proton leakage, protect mitochondria, down-regulate ROS expression, and promote generation of cartilage extracellular matrix. Meanwhile, the system can effectively slow down the progress of osteoarthritis in a rat osteoarthritis model. The micro-nano hydrogel microsphere system for the targeted adjustment and control of the cell MRC function, provided by the invention, has great potential in treating various degenerative diseases related to MRC dysfunction.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering materials and relates to a hydrogel microsphere, in particular to a micro-nano hydrogel microsphere for targeting and regulating the mitochondrial respiratory chain and its preparation and application. Background technique [0002] Mitochondria are important organelles in eukaryotic cells. They regulate energy metabolism and cell cycle through multiple pathways such as apoptosis and autophagy. They are the "energy factories" of eukaryotic cells. The mitochondrial respiratory chain (MRC) is a process in which electron transport (ETC) is coupled with adenosine triphosphate (ATP) synthesis, which takes place in the inner mitochondrial membrane (IMM). When cells are affected by multiple factors such as aging and inflammation, electron leakage occurs in the electron transfer of the mitochondrial respiratory chain, and the leaked electrons directly form oxygen free radicals with oxygen mo...

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Problems solved by technology

However, in the joint cavity of OA patients, because the inflammatory cells of the cartilage secrete a variety of inflammatory factors and metalloproteinases (MMPs), the synovial tissue is in a state of inflammation and swelling, and it is easy to be damaged when the polypeptide and drugs are directly exposed in the joint cavity. Vascular clearing metabolism of inflamed synovium, often requiring multiple applications to achieve therapeutic effect

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