Preparation method of facial skin anti-aging nutrient solution freeze-dried powder as well as product and application thereof

Abstract

The invention provides a preparation method of facial skin anti-aging nutrient solution freeze-dried powder as well as a product and application of the facial skin anti-aging nutrient solution freeze-dried powder. The anti-aging nutrient solution freeze-dried powder is prepared by compounding an umbilical cord-derived mesenchymal stem cell factor extracting solution and nutritional ingredients and then performing freeze-drying. The stem cell factor extracting solution is obtained by carrying out cell lysis on umbilical cord mesenchymal stem cells obtained by culture and centrifugally collecting supernate, contains multiple cell factors, and can play anti-aging and skincare effects. According to the method, the animal-source-free serum culture medium is used for carrying out multiplication culture on the umbilical cord mesenchymal stem cells, and then cell lysis substances are collected, so that the safety of the product is improved on the premise of ensuring the effectiveness.

Description

technical field [0001] The present invention relates to the technical field of cosmetic medicine, in particular to a preparation method of freeze-dried powder of facial skin anti-aging nutrient solution, its product and application, and a nutrient solution for facial skin anti-aging. Background technique [0002] The skin is located in the outermost layer of the body and is in direct contact with the external environment. It can prevent external stimuli such as microbial invasion, chemical composition hazards, and ultraviolet rays. It is the body's first line of defense. Due to the natural growth of age, inadequate daily care, inappropriate or excessive use of cosmetics, excessive fatigue and various accidents, many people have premature skin aging. Especially for facial skin, the symptoms include dry and rough skin, sagging, increased wrinkles and spots, thinning of the stratum corneum, and susceptibility to allergies, etc. However, there are few effective anti-aging skin p...

AI Technical Summary

Problems solved by technology

However, most of the culture media used in traditional mesenchymal stem cell culture methods contain animal serum, which is unsafe when used

Traditional mesenchymal stem cell culture methods also have disadvantages such as long passage period and low effective passage. For example, umbilical cord mesenchymal stem cells are cultured in DMEM-F12 medium containing 10% FBS, with a passage period of 3-5 days and a passage ratio of 1 :2 or 1:3, the effective generation is about 15 generations

In addition, most of the current cytokines come from the stem cell culture medium, and the cell contents including cytokines and other active ingredients have not been effectively released into the culture medium

At present, most anti-aging products with cytokines as the main active ingredients exist in the form of liquid or emulsion, which is not conducive to storage, and it is urgent to develop new dosage forms suitable for long-term storage

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